3P7O
Rat Insulin Degrading Enzyme (Insulysin) E111F mutant with two bound peptides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-08-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.520, 70.910, 114.360 |
| Unit cell angles | 90.00, 92.97, 90.00 |
Refinement procedure
| Resolution | 28.840 - 2.142 |
| R-factor | 0.2154 |
| Rwork | 0.208 |
| R-free | 0.28460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.158 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.088 | 0.410 |
| Number of reflections | 47233 | |
| <I/σ(I)> | 14.96 | 3.98 |
| Completeness [%] | 98.7 | 99.6 |
| Redundancy | 2.8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100 mM sodium citrate pH 6.5, 100 mM ammonium acetate, 20% PEG 4000, 8 mg/ml protein, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
