3P57

Crystal structure of the p300 TAZ2 domain bound to MEF2 on DNA

3P57 の概要

• エントリーDOI: 10.2210/pdb3p57/pdb
• 分子名称: Myocyte-specific enhancer factor 2A, DNA (5'-D(*A*AP*AP*CP*TP*AP*TP*TP*TP*AP*TP*AP*AP*GP*A)-3'), DNA (5'-D(*TP*TP*CP*TP*TP*AP*TP*AP*AP*AP*TP*AP*GP*TP*T)-3'), ... (6 entities in total)
• 機能のキーワード: protein-dna complex, transcription factor, transcriptional activation, p300, zinc finger, transferase-transcription activator-dna complex, transferase/transcription activator/dna
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 13
• 化学式量合計: 104442.81

構造登録者

He, J.,Ye, J.,Riquelme, C.,Liu, J.O. (登録日: 2010-10-08, 公開日: 2011-08-10, 最終更新日: 2024-11-20)

主引用文献

He, J.,Ye, J.,Cai, Y.,Riquelme, C.,Liu, J.O.,Liu, X.,Han, A.,Chen, L.
Structure of p300 bound to MEF2 on DNA reveals a mechanism of enhanceosome assembly.
Nucleic Acids Res., 39:4464-4474, 2011

PubMed Abstract: Transcription co-activators CBP and p300 are recruited by sequence-specific transcription factors to specific genomic loci to control gene expression. A highly conserved domain in CBP/p300, the TAZ2 domain, mediates direct interaction with a variety of transcription factors including the myocyte enhancer factor 2 (MEF2). Here we report the crystal structure of a ternary complex of the p300 TAZ2 domain bound to MEF2 on DNA at 2.2Å resolution. The structure reveals three MEF2:DNA complexes binding to different sites of the TAZ2 domain. Using structure-guided mutations and a mammalian two-hybrid assay, we show that all three interfaces contribute to the binding of MEF2 to p300, suggesting that p300 may use one of the three interfaces to interact with MEF2 in different cellular contexts and that one p300 can bind three MEF2:DNA complexes simultaneously. These studies, together with previously characterized TAZ2 complexes bound to different transcription factors, demonstrate the potency and versatility of TAZ2 in protein-protein interactions. Our results also support a model wherein p300 promotes the assembly of a higher-order enhanceosome by simultaneous interactions with multiple DNA-bound transcription factors.

PubMed: 21278418
DOI: 10.1093/nar/gkr030

実験手法

X-RAY DIFFRACTION (2.1921 Å)