3OTC
Crystal structure of human tRNAHis guanylyltransferase (Thg1)- Native II
Summary for 3OTC
Entry DOI | 10.2210/pdb3otc/pdb |
Related | 3OTB 3OTC 3OTD 3OTE |
Descriptor | tRNA(His) guanylyltransferase (1 entity in total) |
Functional Keywords | guanylyltransferase, polymerase-like palm domain, catalytic carboxylates, transferase |
Biological source | Homo sapiens (human) |
Cellular location | Cytoplasm: Q9NWX6 |
Total number of polymer chains | 2 |
Total formula weight | 63257.88 |
Authors | Hyde, S.J.,Eckenroth, B.E.,Doublie, S. (deposition date: 2010-09-11, release date: 2010-11-17, Last modification date: 2024-02-21) |
Primary citation | Hyde, S.J.,Eckenroth, B.E.,Smith, B.A.,Eberley, W.A.,Heintz, N.H.,Jackman, J.E.,Doublie, S. tRNAHis guanylyltransferase (THG1), a unique 3'-5' nucleotidyl transferase, shares unexpected structural homology with canonical 5'-3' DNA polymerases. Proc.Natl.Acad.Sci.USA, 107:20305-20310, 2010 Cited by PubMed Abstract: All known DNA and RNA polymerases catalyze the formation of phosphodiester bonds in a 5' to 3' direction, suggesting this property is a fundamental feature of maintaining and dispersing genetic information. The tRNA(His) guanylyltransferase (Thg1) is a member of a unique enzyme family whose members catalyze an unprecedented reaction in biology: 3'-5' addition of nucleotides to nucleic acid substrates. The 2.3-Å crystal structure of human THG1 (hTHG1) reported here shows that, despite the lack of sequence similarity, hTHG1 shares unexpected structural homology with canonical 5'-3' DNA polymerases and adenylyl/guanylyl cyclases, two enzyme families known to use a two-metal-ion mechanism for catalysis. The ability of the same structural architecture to catalyze both 5'-3' and 3'-5' reactions raises important questions concerning selection of the 5'-3' mechanism during the evolution of nucleotide polymerases. PubMed: 21059936DOI: 10.1073/pnas.1010436107 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (3.01 Å) |
Structure validation
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