3OTC
Crystal structure of human tRNAHis guanylyltransferase (Thg1)- Native II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 104 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 116.678, 116.678, 104.950 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 3.010 |
Rwork | 0.257 |
R-free | 0.27770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.232 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.120 |
High resolution limit [Å] | 3.010 | 6.480 | 3.010 |
Rmerge | 0.093 | 0.032 | 0.821 |
Number of reflections | 16284 | ||
<I/σ(I)> | 8.4 | ||
Completeness [%] | 99.5 | 100 | 95.5 |
Redundancy | 13.4 | 13.7 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7 | 298 | Na Formate, pH 7, hanging drop, temperature 298K |