3O6E

Crystal Structure of human Hiwi1 PAZ domain (residues 277-399) in complex with 14-mer RNA (12-bp + 2-nt overhang) containing 2'-OCH3 at its 3'-end

Summary for 3O6E

Related3O3I 3O7V 3O7X
DescriptorPiwi-like protein 1, RNA (5'-R(*GP*CP*GP*AP*AP*UP*AP*UP*UP*CP*GP*CP*UP*(OMU))-3') (3 entities in total)
Functional Keywordspiwi, paz, rna silencing, pi-rna, hiwi1, hili, paz domain, rna binding protein-rna complex, rna binding protein/rna
Biological sourceHomo sapiens (human)
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Cellular locationCytoplasm  Q96J94
Total number of polymer chains2
Total molecular weight19063.87
Authors
Tian, Y.,Simanshu, D.K.,Ma, J.-B.,Patel, D.J. (deposition date: 2010-07-28, release date: 2011-01-12, Last modification date: 2017-11-08)
Primary citation
Tian, Y.,Simanshu, D.K.,Ma, J.B.,Patel, D.J.
Inaugural Article: Structural basis for piRNA 2'-O-methylated 3'-end recognition by Piwi PAZ (Piwi/Argonaute/Zwille) domains.
Proc.Natl.Acad.Sci.USA, 108:903-910, 2011
PubMed: 21193640 (PDB entries with the same primary citation)
DOI: 10.1073/pnas.1017762108
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.904 Å)
NMR Information
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.29941.0%03.4%0.43MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 3o6e
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Molmil generated image of 3o6e
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Molmil generated image of 3o6e
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More Biological unit images

Molmil generated image of 3o6e
no rotation
Molmil generated image of 3o6e
rotated about x axis by 90°
Molmil generated image of 3o6e
rotated about y axis by 90°
(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (3o6e.pdb1.gz [97.62 KB])