3O6E

Crystal Structure of human Hiwi1 PAZ domain (residues 277-399) in complex with 14-mer RNA (12-bp + 2-nt overhang) containing 2'-OCH3 at its 3'-end

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 41 2 2
Unit cell lengths	44.013, 44.013, 147.024
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.904
R-factor	0.2562
Rwork	0.252
R-free	0.30510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3o7v
RMSD bond length	0.005
RMSD bond angle	0.842
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.000
High resolution limit [Å]	2.900	6.230	2.900
Rmerge	0.083	0.057	0.209
Number of reflections	3451
<I/σ(I)>	32.5		4.8
Completeness [%]	95.3	99.5	72.3
Redundancy	10.4	10.1	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	1-5% PEG3350, 50 mM NaCl, 50 mM Na-acetate buffer, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K