3O6E
Crystal Structure of human Hiwi1 PAZ domain (residues 277-399) in complex with 14-mer RNA (12-bp + 2-nt overhang) containing 2'-OCH3 at its 3'-end
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 44.013, 44.013, 147.024 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.904 |
R-factor | 0.2562 |
Rwork | 0.252 |
R-free | 0.30510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o7v |
RMSD bond length | 0.005 |
RMSD bond angle | 0.842 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 6.230 | 2.900 |
Rmerge | 0.083 | 0.057 | 0.209 |
Number of reflections | 3451 | ||
<I/σ(I)> | 32.5 | 4.8 | |
Completeness [%] | 95.3 | 99.5 | 72.3 |
Redundancy | 10.4 | 10.1 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 1-5% PEG3350, 50 mM NaCl, 50 mM Na-acetate buffer, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |