3O4L

Genetic and structural basis for selection of a ubiquitous T cell receptor deployed in Epstein-Barr virus

3O4L の概要

• エントリーDOI: 10.2210/pdb3o4l/pdb
• 分子名称: MHC class I antigen, Beta-2-microglobulin, BSLF2/BMLF1 protein, ... (9 entities in total)
• 機能のキーワード: mhc i, immunoglobulin, antigen presentation, immune recognition, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Secreted: P61769
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 95978.47

構造登録者

Miles, J.J.,Bulek, A.M.,Cole, D.K.,Gostick, E.,Schauenburg, J.A.,Dolton, G.,Venturi, V.,Davenport, M.P.,Tan, M.P.,Burrows, S.R.,Wooldridge, L.,Price, D.A.,Rizkallah, P.J.,Sewell, A.K. (登録日: 2010-07-27, 公開日: 2011-01-12, 最終更新日: 2024-11-06)

主引用文献

Miles, J.J.,Bulek, A.M.,Cole, D.K.,Gostick, E.,Schauenburg, A.J.,Dolton, G.,Venturi, V.,Davenport, M.P.,Tan, M.P.,Burrows, S.R.,Wooldridge, L.,Price, D.A.,Rizkallah, P.J.,Sewell, A.K.
Genetic and structural basis for selection of a ubiquitous T cell receptor deployed in Epstein-Barr virus infection.
Plos Pathog., 6:e1001198-e1001198, 2010

PubMed Abstract: Despite the ∼10(18) αβ T cell receptor (TCR) structures that can be randomly manufactured by the human thymus, some surface more frequently than others. The pinnacles of this distortion are public TCRs, which exhibit amino acid-identical structures across different individuals. Public TCRs are thought to result from both recombinatorial bias and antigen-driven selection, but the mechanisms that underlie inter-individual TCR sharing are still largely theoretical. To examine this phenomenon at the atomic level, we solved the co-complex structure of one of the most widespread and numerically frequent public TCRs in the human population. The archetypal AS01 public TCR recognizes an immunodominant BMLF1 peptide, derived from the ubiquitous Epstein-Barr virus, bound to HLA-A*0201. The AS01 TCR was observed to dock in a diagonal fashion, grasping the solvent exposed peptide crest with two sets of complementarity-determining region (CDR) loops, and was fastened to the peptide and HLA-A*0201 platform with residue sets found only within TCR genes biased in the public response. Computer simulations of a random V(D)J recombination process demonstrated that both TCRα and TCRβ amino acid sequences could be manufactured easily, thereby explaining the prevalence of this receptor across different individuals. Interestingly, the AS01 TCR was encoded largely by germline DNA, indicating that the TCR loci already comprise gene segments that specifically recognize this ancient pathogen. Such pattern recognition receptor-like traits within the αβ TCR system further blur the boundaries between the adaptive and innate immune systems.

PubMed: 21124993
DOI: 10.1371/journal.ppat.1001198

実験手法

X-RAY DIFFRACTION (2.54 Å)