3NBN

Crystal structure of a dimer of Notch Transcription Complex trimers on HES1 DNA

3NBN の概要

• エントリーDOI: 10.2210/pdb3nbn/pdb
• 分子名称: Recombining binding protein suppressor of hairless, Neurogenic locus notch homolog protein 1, Mastermind-like protein 1, ... (5 entities in total)
• 機能のキーワード: promoter regions, notch1, csl, rbpj, mastermind, transcription factors, transcription, transcriptional activation, transcription-dna complex, transcription/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: Q06330; Cell membrane ; Single-pass type I membrane protein . Notch 1 intracellular domain: Nucleus : P46531; Nucleus speckle : Q92585
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 192047.56

構造登録者

Arnett, K.L.,Blacklow, S.C. (登録日: 2010-06-03, 公開日: 2010-11-03, 最終更新日: 2024-10-30)

主引用文献

Arnett, K.L.,Hass, M.,McArthur, D.G.,Ilagan, M.X.,Aster, J.C.,Kopan, R.,Blacklow, S.C.
Structural and mechanistic insights into cooperative assembly of dimeric Notch transcription complexes.
Nat.Struct.Mol.Biol., 17:1312-1317, 2010

PubMed Abstract: Ligand-induced proteolysis of Notch produces an intracellular effector domain that transduces essential signals by regulating the transcription of target genes. This function relies on the formation of transcriptional activation complexes that include intracellular Notch, a Mastermind co-activator and the transcription factor CSL bound to cognate DNA. These complexes form higher-order assemblies on paired, head-to-head CSL recognition sites. Here we report the X-ray structure of a dimeric human Notch1 transcription complex loaded on the paired site from the human HES1 promoter. The small interface between the Notch ankyrin domains could accommodate DNA bending and untwisting to allow a range of spacer lengths between the two sites. Cooperative dimerization occurred on the human and mouse Hes5 promoters at a sequence that diverged from the CSL-binding consensus at one of the sites. These studies reveal how promoter organizational features control cooperativity and, thus, the responsiveness of different promoters to Notch signaling.

PubMed: 20972443
DOI: 10.1038/nsmb.1938

実験手法

X-RAY DIFFRACTION (3.45 Å)