3N9K

F229A/E292S Double Mutant of Exo-beta-1,3-glucanase from Candida albicans in Complex with Laminaritriose at 1.7 A

Summary for 3N9K

Related1CZ1 1EQC 2PBO 2PB1 2PF0 2PC8
DescriptorGlucan 1,3-beta-glucosidase, CALCIUM ION, BETA-D-GLUCOSE, ... (4 entities in total)
Functional Keywordsaromatic entranceway/clamp, exoglucanase, glycoside hydrolase, protein-carbohydrate interaction, site-directed mutagenesis, laminaritriose, hydrolase
Biological sourceCandida albicans (Yeast)
Cellular locationSecreted P29717
Total number of polymer chains1
Total molecular weight46558.08
Authors
Nakatani, Y.,Cutfield, S.M.,Cutfield, J.F. (deposition date: 2010-05-30, release date: 2010-09-15, Last modification date: 2011-07-13)
Primary citation
Patrick, W.M.,Nakatani, Y.,Cutfield, S.M.,Sharpe, M.L.,Ramsay, R.J.,Cutfield, J.F.
Carbohydrate binding sites in Candida albicans exo-beta-1,3-glucanase and the role of the Phe-Phe 'clamp' at the active site entrance
Febs J., 277:4549-4561, 2010
PubMed: 20875088 (PDB entries with the same primary citation)
DOI: 10.1111/j.1742-4658.2010.07869.x
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.7 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.19530.3%1.2%1.8%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

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