3N9K
F229A/E292S Double Mutant of Exo-beta-1,3-glucanase from Candida albicans in Complex with Laminaritriose at 1.7 A
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0000272 | biological_process | polysaccharide catabolic process |
A | 0004338 | molecular_function | glucan exo-1,3-beta-glucosidase activity |
A | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
A | 0005576 | cellular_component | extracellular region |
A | 0005975 | biological_process | carbohydrate metabolic process |
A | 0006696 | biological_process | ergosterol biosynthetic process |
A | 0007155 | biological_process | cell adhesion |
A | 0009251 | biological_process | glucan catabolic process |
A | 0009986 | cellular_component | cell surface |
A | 0016740 | molecular_function | transferase activity |
A | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
A | 0031505 | biological_process | fungal-type cell wall organization |
A | 0031589 | biological_process | cell-substrate adhesion |
A | 0044011 | biological_process | single-species biofilm formation on inanimate substrate |
A | 0044042 | biological_process | glucan metabolic process |
A | 0044407 | biological_process | single-species biofilm formation in or on host organism |
A | 0050839 | molecular_function | cell adhesion molecule binding |
A | 0071555 | biological_process | cell wall organization |
A | 1903561 | cellular_component | extracellular vesicle |
Functional Information from PROSITE/UniProt
site_id | PS00659 |
Number of Residues | 10 |
Details | GLYCOSYL_HYDROL_F5 Glycosyl hydrolases family 5 signature. IGIELLNEPL |
Chain | Residue | Details |
A | ILE185-LEU194 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 1 |
Details | ACT_SITE: Proton donor => ECO:0000250|UniProtKB:O85465 |
Chain | Residue | Details |
A | GLU192 |
site_id | SWS_FT_FI2 |
Number of Residues | 1 |
Details | ACT_SITE: Nucleophile => ECO:0000269|PubMed:9013549 |
Chain | Residue | Details |
A | SER292 |
site_id | SWS_FT_FI3 |
Number of Residues | 5 |
Details | BINDING: BINDING => ECO:0000269|PubMed:20875088 |
Chain | Residue | Details |
A | GLU27 | |
A | TYR29 | |
A | GLU192 | |
A | TYR255 | |
A | GLU262 |
Catalytic Information from CSA
site_id | MCSA1 |
Number of Residues | 2 |
Details | M-CSA 831 |
Chain | Residue | Details |
A | GLU192 | activator, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
A | SER292 | covalently attached, nucleofuge, nucleophile |