3N9K
F229A/E292S Double Mutant of Exo-beta-1,3-glucanase from Candida albicans in Complex with Laminaritriose at 1.7 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-03-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.725, 64.397, 94.866 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.190 - 1.700 |
| R-factor | 0.15397 |
| Rwork | 0.152 |
| R-free | 0.19681 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cz1 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.943 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.190 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.074 | 0.636 |
| Number of reflections | 40247 | |
| <I/σ(I)> | 14.1 | 2.2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 291 | 0.1M HEPES-KOH, 0.2M CACL2, 19% PEG 8000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
