3MJH

Crystal Structure of Human Rab5A in complex with the C2H2 Zinc Finger of EEA1

Summary for 3MJH

• Entry DOI: 10.2210/pdb3mjh/pdb
• Descriptor: Ras-related protein Rab-5A, Early endosome antigen 1, GUANOSINE-5'-TRIPHOSPHATE, ... (6 entities in total)
• Functional Keywords: protein-zinc finger complex, beta beta alpha fold, beta hairpin, rab5a gtpase, eea1, protein transport
• Biological source: Homo sapiens (human); More
• Cellular location: Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity): P20339; Cytoplasm: Q15075
• Total number of polymer chains: 4
• Total formula weight: 46184.69

Authors

Mishra, A.K.,Eathiraj, S.,Lambright, D.G. (deposition date: 2010-04-12, release date: 2010-05-05, Last modification date: 2024-02-21)

Primary citation

Mishra, A.,Eathiraj, S.,Corvera, S.,Lambright, D.G.
Structural basis for Rab GTPase recognition and endosome tethering by the C2H2 zinc finger of Early Endosomal Autoantigen 1 (EEA1).
Proc.Natl.Acad.Sci.USA, 107:10866-10871, 2010

PubMed Abstract: Regulation of endosomal trafficking by Rab GTPases depends on selective interactions with multivalent effectors, including EEA1 and Rabenosyn-5, which facilitate endosome tethering, sorting, and fusion. Both EEA1 and Rabenosyn-5 contain a distinctive N-terminal C(2)H(2) zinc finger that binds Rab5. How these C(2)H(2) zinc fingers recognize Rab GTPases remains unknown. Here, we report the crystal structure of Rab5A in complex with the EEA1 C(2)H(2) zinc finger. The binding interface involves all elements of the zinc finger as well as a short N-terminal extension but is restricted to the switch and interswitch regions of Rab5. High selectivity for Rab5 and, to a lesser extent Rab22, is observed in quantitative profiles of binding to Rab family GTPases. Although critical determinants are identified in both switch regions, Rab4-to-Rab5 conversion-of-specificity mutants reveal an essential requirement for additional substitutions in the proximal protein core that are predicted to indirectly influence recognition through affects on the structure and conformational stability of the switch regions.

PubMed: 20534488
DOI: 10.1073/pnas.1000843107

Experimental method

X-RAY DIFFRACTION (2.03 Å)