3L5I

Crystal structure of FnIII domains of human GP130 (Domains 4-6)

3L5I の概要

• エントリーDOI: 10.2210/pdb3l5i/pdb
• 関連するPDBエントリー: 3L5H 3L5J
• 分子名称: Interleukin-6 receptor subunit beta, 1,2-ETHANEDIOL, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: cytokine receptor, fibronectin type iii domain, cell membrane, disulfide bond, immunoglobulin domain, membrane, receptor, secreted, transmembrane, immune system
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Isoform 1: Cell membrane ; Single-pass type I membrane protein . Isoform 2: Secreted : P40189
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34247.27

構造登録者

Kershaw, N.J.,Zhang, J.-G.,Garrett, T.P.J.,Czabotar, P.E. (登録日: 2009-12-22, 公開日: 2010-05-12, 最終更新日: 2017-11-01)

主引用文献

Xu, Y.,Kershaw, N.J.,Luo, C.S.,Soo, P.,Pocock, M.J.,Czabotar, P.E.,Hilton, D.J.,Nicola, N.A.,Garrett, T.P.,Zhang, J.G.
Crystal structure of the entire ectodomain of gp130: insights into the molecular assembly of the tall cytokine receptor complexes.
J.Biol.Chem., 285:21214-21218, 2010

PubMed Abstract: gp130 is the shared signal-transducing receptor subunit for the large and important family of interleukin 6-like cytokines. Previous x-ray structures of ligand-receptor complexes of this family lack the three membrane-proximal domains that are essential for signal transduction. Here we report the crystal structure of the entire extracellular portion of human gp130 (domains 1-6, D1-D6) at 3.6 A resolution, in an unliganded form, as well as a higher resolution structure of the membrane-proximal fibronectin type III domains (D4-D6) at 1.9 A. This represents the first atomic resolution structure of the complete ectodomain of any "tall" cytokine receptor. These structures show that other than a reorientation of the D1 domain, there is little structural change in gp130 upon ligand binding. They also reveal that the interface between the D4 and D5 domains forms an acute bend in the gp130 structure. Key residues at this interface are highly conserved across the entire tall receptor family, suggesting that this acute bend may be a common feature of these receptors. Importantly, this geometry positions the C termini of the membrane-proximal fibronectin type III domains of the tall cytokine receptors in close proximity within the transmembrane complex, favorable for receptor-associated Janus kinases to trans-phosphorylate and activate each other.

PubMed: 20489211
DOI: 10.1074/jbc.C110.129502

実験手法

X-RAY DIFFRACTION (1.9 Å)