3L5I
Crystal structure of FnIII domains of human GP130 (Domains 4-6)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9798, 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.565, 75.347, 107.185 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.753 - 1.900 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.22200 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.972 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.108 | 0.572 |
| Number of reflections | 27278 | |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 99.8 | 98.2 |
| Redundancy | 18.5 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 8000, NaCl, Tris-HCl, pH 8.5, vapor diffusion, hanging drop, temperature 293K |
