Crystal structure of the I93M mutant of ubiquitin carboxy terminal hydrolase L1 bound to ubiquitin vinylmethylester

Summary for 3KVF

Related2ETL 3KW5
DescriptorUbiquitin carboxyl-terminal hydrolase isozyme L1, Ubiquitin, METHYL 4-AMINOBUTANOATE, ... (4 entities in total)
Functional Keywordsenzyme-suicide substrate complex, cytoplasm, disease mutation, glycoprotein, hydrolase, ligase, oxidation, polymorphism, protease, thiol protease, ubl conjugation pathway, isopeptide bond, nucleus, phosphoprotein, ubl conjugation, hydrolase-hydrolase inhibitor complex, acetylation, hydrolase-signaling protein complex, hydrolase/signaling protein
Biological sourceHomo sapiens (human)
Cellular locationCytoplasm P09936
Total number of polymer chains2
Total molecular weight33921.76
Davies, C.W.,Maiti, T.K.,Das, C. (deposition date: 2009-11-30, release date: 2010-06-16, Last modification date: 2011-07-13)
Primary citation
Boudreaux, D.A.,Maiti, T.K.,Davies, C.W.,Das, C.
Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation.
Proc.Natl.Acad.Sci.USA, 107:9117-9122, 2010
PubMed: 20439756 (PDB entries with the same primary citation)
DOI: 10.1073/pnas.0910870107
MImport into Mendeley
Experimental method
NMR Information

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.29740.7%3.5%1.0%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report