3KVF
Crystal structure of the I93M mutant of ubiquitin carboxy terminal hydrolase L1 bound to ubiquitin vinylmethylester
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 87.089, 87.089, 193.476 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.720 - 2.800 |
| R-factor | 0.2442 |
| Rwork | 0.242 |
| R-free | 0.28407 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ifw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.888 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.200 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.089 | 0.856 |
| Number of reflections | 6996 | |
| <I/σ(I)> | 21.7 | 2.1 |
| Completeness [%] | 96.7 | 99.4 |
| Redundancy | 10.5 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 0.1M BICINE, 2.4M Ammonium Sulfate, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
