3KS8

Crystal structure of Reston ebolavirus VP35 RNA binding domain in complex with 18bp dsRNA

3KS8 の概要

• エントリーDOI: 10.2210/pdb3ks8/pdb
• 関連するPDBエントリー: 3FKE 3KS4
• 分子名称: 5'-R(*AP*GP*AP*AP*GP*GP*AP*GP*GP*GP*AP*GP*GP*GP*AP*GP*GP*A)-3', 5'-R(*UP*CP*CP*UP*CP*CP*CP*UP*CP*CP*CP*UP*CP*CP*UP*UP*CP*U)-3', Polymerase cofactor VP35, ... (4 entities in total)
• 機能のキーワード: ebolavirus, rna-binding protein, protein-rna complex, interferon, reston, host cytoplasm, interferon antiviral system evasion, rna replication, rna-binding, transcription, virion, viral protein-rna complex, viral protein/rna
• 由来する生物種: Reston ebolavirus (REBOV)
• 細胞内の位置: Virion: Q8JPY0
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 93789.65

構造登録者

Kimberlin, C.R.,Bornholdt, Z.A.,Li, S.,Woods, V.L.,Macrae, I.J.,Saphire, E.O. (登録日: 2009-11-20, 公開日: 2010-01-12, 最終更新日: 2023-09-06)

主引用文献

Kimberlin, C.R.,Bornholdt, Z.A.,Li, S.,Woods, V.L.,Macrae, I.J.,Saphire, E.O.
Ebolavirus VP35 uses a bimodal strategy to bind dsRNA for innate immune suppression.
Proc.Natl.Acad.Sci.USA, 107:314-319, 2009

PubMed Abstract: Ebolavirus causes a severe hemorrhagic fever and is divided into five distinct species, of which Reston ebolavirus is uniquely nonpathogenic to humans. Disease caused by ebolavirus is marked by early immunosuppression of innate immune signaling events, involving silencing and sequestration of double-stranded RNA (dsRNA) by the viral protein VP35. Here we present unbound and dsRNA-bound crystal structures of the dsRNA-binding domain of Reston ebolavirus VP35. The structures show that VP35 forms an unusual, asymmetric dimer on dsRNA binding, with each of the monomers binding dsRNA in a different way: one binds the backbone whereas the other caps the terminus. Additional SAXS, DXMS, and dsRNA-binding experiments presented here support a model of cooperative dsRNA recognition in which binding of the first monomer assists binding of the next monomer of the oligomeric VP35 protein. This work illustrates how ebolavirus VP35 could mask key recognition sites of molecules such as RIG-I, MDA-5, and Dicer to silence viral dsRNA in infection.

PubMed: 20018665
DOI: 10.1073/pnas.0910547107

実験手法

X-RAY DIFFRACTION (2.401 Å)