3KHU

Crystal structure of human UDP-glucose dehydrogenase Glu161Gln, in complex with thiohemiacetal intermediate

3KHU の概要

• エントリーDOI: 10.2210/pdb3khu/pdb
• 関連するPDBエントリー: 2Q3E 2QG4 3ITK
• 分子名称: UDP-glucose 6-dehydrogenase, URIDINE-5'-DIPHOSPHATE-GLUCOSE, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: oxidoreductase, thiohemiacetal intermediate, structural genomics, structural genomics consortium, sgc
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 320583.69

構造登録者

Chaikuad, A.,Egger, S.,Yue, W.W.,Guo, K.,Sethi, R.,Filippakopoulos, P.,Muniz, J.R.C.,von Delft, F.,Bountra, C.,Arrowsmith, C.H.,Weigelt, J.,Edwards, A.M.,Kavanagh, K.L.,Nidetzky, B.,Oppermann, U.,Structural Genomics Consortium (SGC) (登録日: 2009-10-30, 公開日: 2009-11-17, 最終更新日: 2024-11-06)

主引用文献

Egger, S.,Chaikuad, A.,Klimacek, M.,Kavanagh, K.L.,Oppermann, U.,Nidetzky, B.
Structural and Kinetic Evidence That Catalytic Reaction of Human UDP-glucose 6-Dehydrogenase Involves Covalent Thiohemiacetal and Thioester Enzyme Intermediates.
J.Biol.Chem., 287:2119-2129, 2012

PubMed Abstract: Biosynthesis of UDP-glucuronic acid by UDP-glucose 6-dehydrogenase (UGDH) occurs through the four-electron oxidation of the UDP-glucose C6 primary alcohol in two NAD(+)-dependent steps. The catalytic reaction of UGDH is thought to involve a Cys nucleophile that promotes formation of a thiohemiacetal enzyme intermediate in the course of the first oxidation step. The thiohemiacetal undergoes further oxidation into a thioester, and hydrolysis of the thioester completes the catalytic cycle. Herein we present crystallographic and kinetic evidence for the human form of UGDH that clarifies participation of covalent catalysis in the enzymatic mechanism. Substitution of the putative catalytic base for water attack on the thioester (Glu(161)) by an incompetent analog (Gln(161)) gave a UGDH variant (E161Q) in which the hydrolysis step had become completely rate-limiting so that a thioester enzyme intermediate accumulated at steady state. By crystallizing E161Q in the presence of 5 mm UDP-glucose and 2 mm NAD(+), we succeeded in trapping a thiohemiacetal enzyme intermediate and determined its structure at 2.3 Å resolution. Cys(276) was covalently modified in the structure, establishing its role as catalytic nucleophile of the reaction. The thiohemiacetal reactive C6 was in a position suitable to become further oxidized by hydride transfer to NAD(+). The proposed catalytic mechanism of human UGDH involves Lys(220) as general base for UDP-glucose alcohol oxidation and for oxyanion stabilization during formation and breakdown of the thiohemiacetal and thioester enzyme intermediates. Water coordinated to Asp(280) deprotonates Cys(276) to function as an aldehyde trap and also provides oxyanion stabilization. Glu(161) is the Brønsted base catalytically promoting the thioester hydrolysis.

PubMed: 22123821
DOI: 10.1074/jbc.M111.313015

実験手法

X-RAY DIFFRACTION (2.3 Å)