3KHG

Dpo4 extension ternary complex with misinserted A opposite the 2-aminofluorene-guanine [AF]G lesion

Summary for 3KHG

Related3KHH 3KHL 3KHR
DescriptorDNA polymerase IV, 5'-D(*GP*TP*TP*GP*GP*AP*TP*GP*GP*TP*AP*GP*(2DA))-3', 5'-D(*C*CP*TP*AP*AP*CP*GP*CP*TP*AP*CP*CP*AP*TP*CP*CP*AP*AP*CP*C)-3', ... (8 entities in total)
Functional Keywordslesion bypass, dna polymerase, y-family polymerase, 2-aminofluorene, semi-targeted mutagenesis, dna damage, dna repair, dna replication, dna-binding, dna-directed dna polymerase, magnesium, metal-binding, mutator protein, nucleotidyltransferase, transferase, transferase-dna complex, transferase/dna
Biological sourceSulfolobus solfataricus P2
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Cellular locationCytoplasm (Probable) Q97W02
Total number of polymer chains6
Total molecular weight99245.1
Authors
Rechkoblit, O.,Malinina, L.,Patel, D.J. (deposition date: 2009-10-30, release date: 2010-02-16, Last modification date: 2011-07-13)
Primary citation
Rechkoblit, O.,Kolbanovskiy, A.,Malinina, L.,Geacintov, N.E.,Broyde, S.,Patel, D.J.
Mechanism of error-free and semitargeted mutagenic bypass of an aromatic amine lesion by Y-family polymerase Dpo4.
Nat.Struct.Mol.Biol., 17:379-388, 2010
PubMed: 20154704 (PDB entries with the same primary citation)
DOI: 10.1038/nsmb.1771
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.96 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.27792.2%7.2%3.8%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload