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3K05

The crystal structure of MDC1 BRCT T2067D in complex with a minimal recognition tetrapeptide with an amidated C-terminus

Summary for 3K05
Entry DOI10.2210/pdb3k05/pdb
Related3K0H 3K0K
DescriptorMediator of DNA damage checkpoint protein 1, phospho peptide, GLYCEROL, ... (4 entities in total)
Functional Keywordsbrct domain, protein-peptide complex, phospho protein binding, h2ax, dna damage response, cell cycle, dna damage, dna repair, nucleus, phosphoprotein, protein binding
Biological sourceHomo sapiens (human)
More
Cellular locationNucleus : Q14676
Total number of polymer chains4
Total formula weight45287.88
Authors
Campbell, S.J.,Edwards, R.A.,Glover, J.N. (deposition date: 2009-09-24, release date: 2010-03-02, Last modification date: 2024-11-20)
Primary citationCampbell, S.J.,Edwards, R.A.,Glover, J.N.
Comparison of the Structures and Peptide Binding Specificities of the BRCT Domains of MDC1 and BRCA1
Structure, 18:167-176, 2010
Cited by
PubMed Abstract: The tandem BRCT domains of BRCA1 and MDC1 facilitate protein signaling at DNA damage foci through specific interactions with serine-phosphorylated protein partners. The MDC1 BRCT binds pSer-Gln-Glu-Tyr-COO(-) at the C terminus of the histone variant gammaH2AX via direct recognition of the C-terminal carboxylate, while BRCA1 recognizes pSer-X-X-Phe motifs either at C-terminal or internal sites within target proteins. Using fluorescence polarization binding assays, we show that while both BRCTs prefer a free main chain carboxylate at the +3 position, this preference is much more pronounced in MDC1. Crystal structures of BRCA1 and MDC1 bound to tetrapeptide substrates reveal differences in the environment of conserved arginines (Arg1699 in BRCA1 and Arg1933 in MDC1) that determine the relative affinity for peptides with -COO(-) versus -CO-NH(2) termini. A mutation in MDC1 that induces a more BRCA1-like conformation relaxes the binding specificity, allowing the mutant to bind phosphopeptides lacking a -COO(-) terminus.
PubMed: 20159462
DOI: 10.1016/j.str.2009.12.008
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.33 Å)
Structure validation

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