3JAB
Domain organization and conformational plasticity of the G protein effector, PDE6
Summary for 3JAB
| Entry DOI | 10.2210/pdb3jab/pdb |
| EMDB information | 6258 |
| Descriptor | GafA domain of cone phosphodiesterase 6C, GafB domain of phosphodiesterase 2A, phosphodiesterase 5/6 chimera catalytic domain, ... (7 entities in total) |
| Functional Keywords | phosphodiesterase, photoreceptor, hydrolase-immune system complex, hydrolase/immune system |
| Biological source | Bos taurus (bovine) More |
| Total number of polymer chains | 12 |
| Total formula weight | 259457.92 |
| Authors | Zhang, Z.,He, F.,Constantine, R.,Baker, M.L.,Baehr, W.,Schmid, M.F.,Wensel, T.G.,Agosto, M.A. (deposition date: 2015-05-26, release date: 2015-06-10, Last modification date: 2024-10-16) |
| Primary citation | Zhang, Z.,He, F.,Constantine, R.,Baker, M.L.,Baehr, W.,Schmid, M.F.,Wensel, T.G.,Agosto, M.A. Domain Organization and Conformational Plasticity of the G Protein Effector, PDE6. J.Biol.Chem., 290:12833-12843, 2015 Cited by PubMed Abstract: The cGMP phosphodiesterase of rod photoreceptor cells, PDE6, is the key effector enzyme in phototransduction. Two large catalytic subunits, PDE6α and -β, each contain one catalytic domain and two non-catalytic GAF domains, whereas two small inhibitory PDE6γ subunits allow tight regulation by the G protein transducin. The structure of holo-PDE6 in complex with the ROS-1 antibody Fab fragment was determined by cryo-electron microscopy. The ∼11 Å map revealed previously unseen features of PDE6, and each domain was readily fit with high resolution structures. A structure of PDE6 in complex with prenyl-binding protein (PrBP/δ) indicated the location of the PDE6 C-terminal prenylations. Reconstructions of complexes with Fab fragments bound to N or C termini of PDE6γ revealed that PDE6γ stretches from the catalytic domain at one end of the holoenzyme to the GAF-A domain at the other. Removal of PDE6γ caused dramatic structural rearrangements, which were reversed upon its restoration. PubMed: 25809480DOI: 10.1074/jbc.M115.647636 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (11 Å) |
Structure validation
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