3J5M

Cryo-EM structure of the BG505 SOSIP.664 HIV-1 Env trimer with 3 PGV04 Fabs

3J5M の概要

• エントリーDOI: 10.2210/pdb3j5m/pdb
• 関連するPDBエントリー: 4NCO
• EMDBエントリー: 5779
• 分子名称: BG505 SOSIP gp120, BG505 SOSIP gp41, PGV04 light chain, ... (4 entities in total)
• 機能のキーワード: hiv-1 trimeric spike, gp140, sosip, broadly neutralizing antibody, pgv04, env, envelope glycoprotein, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 318913.28

構造登録者

Lyumkis, D.,Julien, J.-P.,Wilson, I.A.,Ward, A.B. (登録日: 2013-10-26, 公開日: 2013-11-13, 最終更新日: 2024-11-27)

主引用文献

Lyumkis, D.,Julien, J.P.,de Val, N.,Cupo, A.,Potter, C.S.,Klasse, P.J.,Burton, D.R.,Sanders, R.W.,Moore, J.P.,Carragher, B.,Wilson, I.A.,Ward, A.B.
Cryo-EM structure of a fully glycosylated soluble cleaved HIV-1 envelope trimer.
Science, 342:1484-1490, 2013

PubMed Abstract: The HIV-1 envelope glycoprotein (Env) trimer contains the receptor binding sites and membrane fusion machinery that introduce the viral genome into the host cell. As the only target for broadly neutralizing antibodies (bnAbs), Env is a focus for rational vaccine design. We present a cryo-electron microscopy reconstruction and structural model of a cleaved, soluble Env trimer (termed BG505 SOSIP.664 gp140) in complex with a CD4 binding site (CD4bs) bnAb, PGV04, at 5.8 angstrom resolution. The structure reveals the spatial arrangement of Env components, including the V1/V2, V3, HR1, and HR2 domains, as well as shielding glycans. The structure also provides insights into trimer assembly, gp120-gp41 interactions, and the CD4bs epitope cluster for bnAbs, which covers a more extensive area and defines a more complex site of vulnerability than previously described.

PubMed: 24179160
DOI: 10.1126/science.1245627

実験手法

ELECTRON MICROSCOPY (5.8 Å)