3J3X
Independent reconstruction of Mm-cpn cryo-EM density map from half dataset in the closed state (training map)
Summary for 3J3X
| Entry DOI | 10.2210/pdb3j3x/pdb |
| EMDB information | 5645 5646 |
| Descriptor | Chaperonin (1 entity in total) |
| Functional Keywords | modeling, independent reconstruction, cryo-em model validation, chaperone |
| Biological source | Methanococcus maripaludis |
| Total number of polymer chains | 16 |
| Total formula weight | 915389.25 |
| Authors | DiMaio, F.,Zhang, J.,Chiu, W.,Baker, D. (deposition date: 2013-05-02, release date: 2013-05-29, Last modification date: 2024-02-21) |
| Primary citation | Dimaio, F.,Zhang, J.,Chiu, W.,Baker, D. Cryo-EM model validation using independent map reconstructions. Protein Sci., 22:865-868, 2013 Cited by PubMed Abstract: An increasing number of cryo-electron microscopy (cryo-EM) density maps are being generated with suitable resolution to trace the protein backbone and guide sidechain placement. Generating and evaluating atomic models based on such maps would be greatly facilitated by independent validation metrics for assessing the fit of the models to the data. We describe such a metric based on the fit of atomic models with independent test maps from single particle reconstructions not used in model refinement. The metric provides a means to determine the proper balance between the fit to the density and model energy and stereochemistry during refinement, and is likely to be useful in determining values of model building and refinement metaparameters quite generally. PubMed: 23592445DOI: 10.1002/pro.2267 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.3 Å) |
Structure validation
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