3J3X
Independent reconstruction of Mm-cpn cryo-EM density map from half dataset in the closed state (training map)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
| Method | 1 blot 3 seconds |
EM Data Collection
| Microscope | JEOL 3200FSC |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 4.1 |
| Energy Filter | In-column Omega Filter |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.3 |
| Software | EMAN |
| Symmetry | D8 |
