3IOJ

Crystal structure of the Fucosylgalactoside alpha N-acetylgalactosaminyltransferase (GTA, cisAB mutant L266G, G268A) in complex with UDP

Summary for 3IOJ

• Entry DOI: 10.2210/pdb3ioj/pdb
• Related: 1LZ0 3IOH 3IOI
• Descriptor: Histo-blood group ABO system transferase, MANGANESE (II) ION, URIDINE-5'-DIPHOSPHATE, ... (6 entities in total)
• Functional Keywords: gta, abo, cisab mutant, aa(gly)b, rossmann fold, udp, semi-closed conformation, blood group antigen, glycoprotein, glycosyltransferase, golgi apparatus, manganese, membrane, metal-binding, polymorphism, secreted, signal-anchor, transferase, transmembrane
• Biological source: Homo sapiens (human)
• Cellular location: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein: P16442
• Total number of polymer chains: 2
• Total formula weight: 70794.65

Authors

Pesnot, T.,Jorgensen, R.,Palcic, M.M.,Wagner, G.K. (deposition date: 2009-08-14, release date: 2010-04-07, Last modification date: 2023-09-06)

Primary citation

Pesnot, T.,Jorgensen, R.,Palcic, M.M.,Wagner, G.K.
Structural and mechanistic basis for a new mode of glycosyltransferase inhibition.
Nat.Chem.Biol., 6:321-323, 2010

PubMed Abstract: Glycosyltransferases are carbohydrate-active enzymes with essential roles in numerous important biological processes. We have developed a new donor analog for galactosyltransferases that locks a representative target enzyme in a catalytically inactive conformation, thus almost completely abolishing sugar transfer. Results with other galactosyltransferases suggest that this unique mode of glycosyltransferase inhibition may also be generally applicable to other members of this important enzyme family.

PubMed: 20364127
DOI: 10.1038/nchembio.343

Experimental method

X-RAY DIFFRACTION (1.651 Å)