3IOJ
Crystal structure of the Fucosylgalactoside alpha N-acetylgalactosaminyltransferase (GTA, cisAB mutant L266G, G268A) in complex with UDP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Histo-blood group ABO system transferase | polymer | 298 | 34654.0 | 2 | UniProt (P16442) Pfam (PF03414) | Homo sapiens (human) | NAGAT, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Histo-blood group A transferase, A transferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, Histo-blood group B transferase, B transferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form |
| 2 | C, I (A, B) | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) PubChem (27854) | |||
| 3 | D, J (A, B) | URIDINE-5'-DIPHOSPHATE | non-polymer | 404.2 | 2 | Chemie (UDP) PubChem (1158) PubChem (6031) PubChem (25323044) PubChem (25323048) PubChem (44298965) PubChem (53486216) PubChem (60098857) PubChem (57448939) PubChem (3084486) PubChem (91746160) PubChem (6101632) PubChem (92332560) PubChem (92332561) PubChem (92332562) PubChem (92332563) PubChem (93088131) PubChem (93630083) PubChem (6604175) PubChem (11811538) PubChem (12049241) | |||
| 4 | E, K (A, B) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) PubChem (753) | |||
| 5 | F, G, H, L (A, B) | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
| 6 | M, N (A, B) | water | water | 18.0 | 524 | Chemie (HOH) |
Sequence modifications
A, B: 64 - 354 (UniProt: P16442)
| PDB | External Database | Details |
|---|---|---|
| Met 57 | - | expression tag |
| Ala 58 | - | expression tag |
| Ile 59 | - | expression tag |
| Gly 60 | - | expression tag |
| Glu 61 | - | expression tag |
| Phe 62 | - | expression tag |
| Met 63 | - | expression tag |
| Gly 266 | Leu 266 | engineered mutation |
| Ala 268 | Gly 268 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 69308.0 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1486.6 | |
| All* | Total formula weight | 70794.7 |
