3IN5

Structure of human DNA polymerase kappa inserting dATP opposite an 8-oxoG DNA lesion

Replaces:  3HED

Summary for 3IN5

• Entry DOI: 10.2210/pdb3in5/pdb
• Related: 1T94 2OH2
• Descriptor: DNA polymerase kappa, DNA (5'-D(*GP*G*GP*GP*GP*AP*AP*GP*GP*AP*CP*TP*(DOC))-3'), DNA (5'-D(*C*CP*TP*AP*(8OG)P*GP*AP*GP*TP*CP*CP*TP*TP*CP*CP*CP*CP*C)-3'), ... (6 entities in total)
• Functional Keywords: alternative splicing, dna damage, dna repair, dna replication, dna synthesis, dna-binding, dna-directed dna polymerase, magnesium, metal-binding, mutator protein, nucleotidyltransferase, nucleus, phosphoprotein, polymorphism, schiff base, transferase, zinc, zinc-finger, transferase-dna complex, transferase/dna
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus: Q9UBT6
• Total number of polymer chains: 6
• Total formula weight: 136128.45

Authors

Silverstein, T.D.,Vasquez-Del Carpio, R.,Aggarwal, A.K. (deposition date: 2009-08-11, release date: 2009-09-08, Last modification date: 2023-09-06)

Primary citation

Vasquez-Del Carpio, R.,Silverstein, T.D.,Lone, S.,Swan, M.K.,Choudhury, J.R.,Johnson, R.E.,Prakash, S.,Prakash, L.,Aggarwal, A.K.
Structure of human DNA polymerase kappa inserting dATP opposite an 8-oxoG DNA lesion
PLOS ONE, 4:e5766-e5766, 2009

PubMed Abstract: Oxygen-free radicals formed during normal aerobic cellular metabolism attack bases in DNA and 7,8-dihydro-8-oxoguanine (8-oxoG) is one of the major lesions formed. It is amongst the most mutagenic lesions in cells because of its dual coding potential, wherein 8-oxoG(syn) can pair with an A in addition to normal base pairing of 8-oxoG(anti) with a C. Human DNA polymerase kappa (Polkappa) is a member of the newly discovered Y-family of DNA polymerases that possess the ability to replicate through DNA lesions. To understand the basis of Polkappa's preference for insertion of an A opposite 8-oxoG lesion, we have solved the structure of Polkappa in ternary complex with a template-primer presenting 8-oxoG in the active site and with dATP as the incoming nucleotide.

PubMed: 19492058
DOI: 10.1371/journal.pone.0005766

Experimental method

X-RAY DIFFRACTION (3.2 Å)