Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

3ID8

Ternary complex of human pancreatic glucokinase crystallized with activator, glucose and AMP-PNP

Summary for 3ID8
Entry DOI10.2210/pdb3id8/pdb
Related1V4S 3F9M 3FGU 3IDH
DescriptorGlucokinase, alpha-D-glucopyranose, 2-AMINO-4-FLUORO-5-[(1-METHYL-1H-IMIDAZOL-2-YL)SULFANYL]-N-(1,3-THIAZOL-2-YL)BENZAMIDE, ... (7 entities in total)
Functional Keywordsglucokinase, hexokinase iv, atp-binding, diabetes mellitus, disease mutation, glycolysis, kinase, nucleotide-binding, transferase
Biological sourceHomo sapiens (human)
Total number of polymer chains1
Total formula weight54095.35
Authors
Petit, P.,Gluais, L.,Lagarde, A.,Boutin, J.A.,Ferry, G.,Vuillard, L. (deposition date: 2009-07-20, release date: 2010-07-28, Last modification date: 2023-11-01)
Primary citationPetit, P.,Antoine, M.,Ferry, G.,Boutin, J.A.,Lagarde, A.,Gluais, L.,Vincentelli, R.,Vuillard, L.
The active conformation of human glucokinase is not altered by allosteric activators
Acta Crystallogr.,Sect.D, 67:929-935, 2011
Cited by
PubMed Abstract: Glucokinase (GK) catalyses the formation of glucose 6-phosphate from glucose and ATP. A specific feature of GK amongst hexokinases is that it can cycle between active and inactive conformations as a function of glucose concentration, resulting in a unique positive kinetic cooperativity with glucose, which turns GK into a unique key sensor of glucose metabolism, notably in the pancreas. GK is a target of antidiabetic drugs aimed at the activation of GK activity, leading to insulin secretion. Here, the first structures of a GK-glucose complex without activator, of GK-glucose-AMP-PNP and of GK-glucose-AMP-PNP with a bound activator are reported. All these structures are extremely similar, thus demonstrating that binding of GK activators does not result in conformational changes of the active protein but in stabilization of the active form of GK.
PubMed: 22101819
DOI: 10.1107/S0907444911036729
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.4 Å)
Structure validation

227344

PDB entries from 2024-11-13

PDB statisticsPDBj update infoContact PDBjnumon