3ID8
Ternary complex of human pancreatic glucokinase crystallized with activator, glucose and AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-11-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.000, 82.600, 86.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.400 |
| R-factor | 0.20512 |
| Rwork | 0.201 |
| R-free | 0.27908 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v4s |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.513 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.940 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 0.420 |
| Number of reflections | 18612 | |
| <I/σ(I)> | 16.8 | 5.2 |
| Completeness [%] | 95.8 | 91.5 |
| Redundancy | 6.3 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
