3HDE

Crystal structure of full-length endolysin R21 from phage 21

Summary for 3HDE

Entry DOI	10.2210/pdb3hde/pdb
Related	3HDF
Descriptor	Lysozyme (2 entities in total)
Functional Keywords	lysozyme-like, antimicrobial, bacteriolytic enzyme, glycosidase, hydrolase, late protein
Biological source	Enterobacteria phage P21 (Bacteriophage 21)
Total number of polymer chains	4
Total formula weight	72159.70
Authors	Sun, Q.,Arockiasamy, A.,McKee, E.,Caronna, E.,Sacchettini, J.C. (deposition date: 2009-05-07, release date: 2009-11-03, Last modification date: 2024-10-30)
Primary citation	Sun, Q.,Kuty, G.F.,Arockiasamy, A.,Xu, M.,Young, R.,Sacchettini, J.C. Regulation of a muralytic enzyme by dynamic membrane topology. Nat.Struct.Mol.Biol., 16:1192-1194, 2009 Cited by PubMed Abstract: R(21), the lysozyme of coliphage 21, has an N-terminal signal-anchor-release (SAR) domain that directs its secretion in a membrane-tethered, inactive form and then its release and activation in the periplasm. Both genetic and crystallographic studies show that the SAR domain, once extracted from the bilayer, refolds into the body of the enzyme and effects muralytic activation by repositioning one residue of the canonical lysozyme catalytic triad. PubMed: 19881499 DOI: 10.1038/nsmb.1681 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.95 Å)

Structure validation