3HDE
Crystal structure of full-length endolysin R21 from phage 21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2008-06-29 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.249, 94.814, 97.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.410 - 1.950 |
Rwork | 0.212 |
R-free | 0.24800 |
Structure solution method | SIRAS |
RMSD bond length | 0.005 |
RMSD bond angle | 0.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.062 | 0.421 |
Number of reflections | 53709 | |
<I/σ(I)> | 45.0787 | 4.0351 |
Completeness [%] | 99.8 | 98.9 |
Redundancy | 7.1 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 290 | 150mM Sodium acetate, 2M sodium formate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |