3E3J

Crystal Structure of an Intermediate Complex of T7 RNAP and 8nt of RNA

Summary for 3E3J

Related3E2E
DescriptorDNA-directed RNA polymerase, DNA (28-MER), DNA (5'-D(*DTP*DAP*DAP*DTP*DAP*DCP*DGP*DAP*DCP*DTP*DCP*DAP*DCP*DTP*DAP*DTP*DAP*DTP*DTP*DTP*DCP*DTP*DGP*DCP*DCP*DAP*DAP*DAP*DCP*DGP*DGP*DC)-3'), ... (4 entities in total)
Functional Keywordst7 rna polymerase, dna-directed rna polymerase, transcription, nucleotidyltransferase, transferase, transferase-rna-dna complex, transferase/rna/dna
Biological sourceBacteriophage T7
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Total number of polymer chains8
Total molecular weight243982.81
Authors
Durniak, K.J.,Bailey, S.,Steitz, T.A. (deposition date: 2008-08-07, release date: 2008-11-04, Last modification date: 2017-10-25)
Primary citation
Durniak, K.J.,Bailey, S.,Steitz, T.A.
The structure of a transcribing t7 RNA polymerase in transition from initiation to elongation
Science, 322:553-557, 2008
PubMed: 18948533 (PDB entries with the same primary citation)
DOI: 10.1126/science.1163433
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (6.7 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.449695.0%4.2%9.0%0.68MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution