3E3J
Crystal Structure of an Intermediate Complex of T7 RNAP and 8nt of RNA
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | C, B | DNA-directed RNA polymerase | polymer | 889 | 99829.2 | 2 | UniProt (P00573) Pfam (PF14700) Pfam (PF00940) In PDB | Bacteriophage T7 | |
2 | T, X | DNA (28-MER) | polymer | 32 | 9788.3 | 2 | synthetic construct | ||
3 | N, Y | DNA (5'-D(*DTP*DAP*DAP*DTP*DAP*DCP*DGP*DAP*DCP*DTP*DCP*DAP*DCP*DTP*DAP*DTP*DAP*DTP*DTP*DTP*DCP*DTP*DGP*DCP*DCP*DAP*DAP*DAP*DCP*DGP*DGP*DC)-3') | polymer | 32 | 9744.3 | 2 | synthetic construct | ||
4 | R, Z | RNA (5'-R(*GP*GP*GP*AP*GP*UP*AP*A)-3') | polymer | 8 | 2629.6 | 2 | synthetic construct |
Sequence modifications
C, B: 1 - 883 (UniProt: P00573)
PDB | External Database | Details |
---|---|---|
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
His -1 | - | expression tag |
His 0 | - | expression tag |
Leu 266 | Pro 266 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 243982.8 | |
All* | Total formula weight | 243982.8 |