3E3J

Crystal Structure of an Intermediate Complex of T7 RNAP and 8nt of RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (C, B)	DNA-directed RNA polymerase	polymer	889	99829.2	2	UniProt (P00573) Pfam (PF14700) Pfam (PF00940)	Bacteriophage T7
2	B, E (T, X)	DNA (28-MER)	polymer	32	9788.3	2		synthetic construct
3	C, F (N, Y)	DNA (5'-D(*DTP*DAP*DAP*DTP*DAP*DCP*DGP*DAP*DCP*DTP*DCP*DAP*DCP*DTP*DAP*DTP*DAP*DTP*DTP*DTP*DCP*DTP*DGP*DCP*DCP*DAP*DAP*DAP*DCP*DGP*DGP*DC)-3')	polymer	32	9744.3	2		synthetic construct
4	G, H (R, Z)	RNA (5'-R(*GP*GP*GP*AP*GP*UP*AP*A)-3')	polymer	8	2629.6	2		synthetic construct

Sequence modifications

C, B: 1 - 883 (UniProt: P00573)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Leu 266	Pro 266	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	243982.8
All*	Total formula weight	243982.8

*Water molecules are not included.