3E3J
Crystal Structure of an Intermediate Complex of T7 RNAP and 8nt of RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 77.4 |
Detector technology | CCD |
Collection date | 2007-12-10 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 99.216, 180.602, 198.619 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.810 - 6.700 |
R-factor | 0.39486 |
Rwork | 0.392 |
R-free | 0.45419 |
Starting model (for MR) | 3e2e |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 6.940 |
High resolution limit [Å] | 6.700 | 14.330 | 6.700 |
Rmerge | 0.152 | 0.123 | 0.601 |
Number of reflections | 6015 | ||
Completeness [%] | 87.8 | 97.8 | 39.7 |
Redundancy | 3.5 | 4.3 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 285 | PEG 6000, MgCl2, Tris ph8.5 at 285K, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 6000 | ||
2 | 1 | 2 | PEG 6000 | ||
3 | 1 | 1 | MgCl2 | ||
4 | 1 | 2 | MgCl2 | ||
5 | 1 | 1 | Tris | ||
6 | 1 | 2 | Tris |