3DKC

Structure of MET receptor tyrosine kinase in complex with ATP

Summary for 3DKC

• Entry DOI: 10.2210/pdb3dkc/pdb
• Related: 3DKF 3DKG
• Descriptor: Hepatocyte growth factor receptor, MAGNESIUM ION, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: drug discovery, c-met kinase, fragment based, membrane, receptor, transmembrane, oncoprotein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 36317.29

Authors

Hendle, J. (deposition date: 2008-06-24, release date: 2009-07-07, Last modification date: 2024-02-21)

Primary citation

Buchanan, S.G.,Hendle, J.,Lee, P.S.,Smith, C.R.,Bounaud, P.Y.,Jessen, K.A.,Tang, C.M.,Huser, N.H.,Felce, J.D.,Froning, K.J.,Peterman, M.C.,Aubol, B.E.,Gessert, S.F.,Sauder, J.M.,Schwinn, K.D.,Russell, M.,Rooney, I.A.,Adams, J.,Leon, B.C.,Do, T.H.,Blaney, J.M.,Sprengeler, P.A.,Thompson, D.A.,Smyth, L.,Pelletier, L.A.,Atwell, S.,Holme, K.,Wasserman, S.R.,Emtage, S.,Burley, S.K.,Reich, S.H.
SGX523 is an exquisitely selective, ATP-competitive inhibitor of the MET receptor tyrosine kinase with antitumor activity in vivo.
Mol.Cancer Ther., 8:3181-3190, 2009

PubMed Abstract: The MET receptor tyrosine kinase has emerged as an important target for the development of novel cancer therapeutics. Activation of MET by mutation or gene amplification has been linked to kidney, gastric, and lung cancers. In other cancers, such as glioblastoma, autocrine activation of MET has been demonstrated. Several classes of ATP-competitive inhibitor have been described, which inhibit MET but also other kinases. Here, we describe SGX523, a novel, ATP-competitive kinase inhibitor remarkable for its exquisite selectivity for MET. SGX523 potently inhibited MET with an IC50 of 4 nmol/L and is >1,000-fold selective versus the >200-fold selectivity of other protein kinases tested in biochemical assays. Crystallographic study revealed that SGX523 stabilizes MET in a unique inactive conformation that is inaccessible to other protein kinases, suggesting an explanation for the selectivity. SGX523 inhibited MET-mediated signaling, cell proliferation, and cell migration at nanomolar concentrations but had no effect on signaling dependent on other protein kinases, including the closely related RON, even at micromolar concentrations. SGX523 inhibition of MET in vivo was associated with the dose-dependent inhibition of growth of tumor xenografts derived from human glioblastoma and lung and gastric cancers, confirming the dependence of these tumors on MET catalytic activity. Our results show that SGX523 is the most selective inhibitor of MET catalytic activity described to date and is thus a useful tool to investigate the role of MET kinase in cancer without the confounding effects of promiscuous protein kinase inhibition.

PubMed: 19934279
DOI: 10.1158/1535-7163.MCT-09-0477

Experimental method

X-RAY DIFFRACTION (1.52 Å)