3DKC

Structure of MET receptor tyrosine kinase in complex with ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-08
Detector	MAR CCD 165 mm
Wavelength(s)	0.97969
Spacegroup name	P 21 21 21
Unit cell lengths	43.027, 45.083, 167.264
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.100 - 1.520
Rwork	0.189
R-free	0.21800
Data scaling software	SCALA (3.2.5)
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	21.115
High resolution limit [Å]	1.520
Rmerge	0.068
Number of reflections	50425
<I/σ(I)>	7.7
Completeness [%]	98.6
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.2	292	11% ISOPROPANOL, 2.5% PEG 5K MME, 100 mM BIS-TRIS, pH 6.2, VAPOR DIFFUSION, temperature 292K