3DEG

Complex of elongating Escherichia coli 70S ribosome and EF4(LepA)-GMPPNP

Summary for 3DEG

EMDB information1524
DescriptorA/L-tRNA, P-tRNA, 30S RNA helix 8, ... (11 entities in total)
Functional Keywordsribosome, translation, lepa, ef4, gtp-binding, membrane, nucleotide-binding, antibiotic resistance, ribonucleoprotein, ribosomal protein, rna-binding, rrna-binding, trna-binding, methylation
Biological sourceEscherichia coli
Cellular locationCell inner membrane; Peripheral membrane protein; Cytoplasmic side (Probable) P60785
Total number of polymer chains11
Total molecular weight190058.63
Authors
Connell, S.R.,Topf, M.,Qin, Y.,Wilson, D.N.,Mielke, T.,Fucini, P.,Nierhaus, K.H.,Spahn, C.M.T. (deposition date: 2008-06-10, release date: 2008-08-19, Last modification date: 2014-04-16)
Primary citation
Connell, S.R.,Topf, M.,Qin, Y.,Wilson, D.N.,Mielke, T.,Fucini, P.,Nierhaus, K.H.,Spahn, C.M.T.
A new tRNA intermediate revealed on the ribosome during EF4-mediated back-translocation
Nat.Struct.Mol.Biol., 15:910-915, 2008
PubMed: 19172743 (PDB entries with the same primary citation)
DOI: 10.1038/nsmb.1469
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
?

Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone393.0%12.0%0.57MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

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