3DEG
Complex of elongating Escherichia coli 70S ribosome and EF4(LepA)-GMPPNP
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | KODAK SO-163 FILM |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 10.9 |
| Software | Flex-EM,Mod-EM,Situs,SPIDER |
| Symmetry | C1 |
