3CZJ
E. COLI (lacZ) BETA-GALACTOSIDASE (N460T) IN COMPLEX WITH D-GALCTOPYRANOSYL-1-ONE
Summary for 3CZJ
Entry DOI | 10.2210/pdb3czj/pdb |
Related | 1DP0 |
Descriptor | Beta-galactosidase, D-galactonolactone, MAGNESIUM ION, ... (6 entities in total) |
Functional Keywords | asn-460-thr beta-galactosidase hydrolase tim barrel (alpha/beta barrel) jelly-roll barrel immunoglobulin beta supersandwich, glycosidase, hydrolase |
Biological source | Escherichia coli |
Total number of polymer chains | 4 |
Total formula weight | 474616.71 |
Authors | Huber, R.E.,Dugdale, M.L.,Fraser, M.E.,Tammam, S.D. (deposition date: 2008-04-29, release date: 2009-04-07, Last modification date: 2023-08-30) |
Primary citation | Kappelhoff, J.C.,Liu, S.Y.,Dugdale, M.L.,Dymianiw, D.L.,Linton, L.R.,Huber, R.E. Practical Considerations When Using Temperature to Obtain Rate Constants and Activation Thermodynamics of Enzymes with Two Catalytic Steps: Native and N460T-beta-Galactosidase (E. coli) as Examples. Protein J., 28:96-103, 2009 Cited by PubMed: 19229596DOI: 10.1007/s10930-009-9168-1 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.05 Å) |
Structure validation
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