3CZJ
E. COLI (lacZ) BETA-GALACTOSIDASE (N460T) IN COMPLEX WITH D-GALCTOPYRANOSYL-1-ONE
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Beta-galactosidase | polymer | 1023 | 116493.3 | 4 | UniProt (P00722) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) | Escherichia coli | Lactase |
| 2 | E, JA, RB, XC (A, B, C, D) | D-galactonolactone | non-polymer | 178.1 | 4 | Chemie (149) | |||
| 3 | F, G, KA, LA, MA... (A, B, C, D) | MAGNESIUM ION | non-polymer | 24.3 | 9 | Chemie (MG) | |||
| 4 | AD, BD, CD, DD, H... (D, A, B, C) | SODIUM ION | non-polymer | 23.0 | 16 | Chemie (NA) | |||
| 5 | AA, AB, AC, BA, BB... (A, B, C, D) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 94 | Chemie (DMS) | |||
| 6 | AE, XD, YD, ZD (D, A, B, C) | water | water | 18.0 | 2607 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 9 - 1023 (UniProt: P00722)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Ser 2 | - | expression tag |
| His 3 | - | expression tag |
| Met 4 | - | expression tag |
| Leu 5 | - | expression tag |
| Glu 6 | - | expression tag |
| Asp 7 | - | expression tag |
| Pro 8 | - | expression tag |
| Thr 460 | Asn 461 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 465973.1 | |
| Non-Polymers* | Number of molecules | 123 |
| Total formula weight | 8643.6 | |
| All* | Total formula weight | 474616.7 |
