3CZJ
E. COLI (lacZ) BETA-GALACTOSIDASE (N460T) IN COMPLEX WITH D-GALCTOPYRANOSYL-1-ONE
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Beta-galactosidase | polymer | 1023 | 116493.3 | 4 | UniProt (P00722) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) In PDB | Escherichia coli | Lactase |
2 | A, B, C, D | D-galactonolactone | non-polymer | 178.1 | 4 | Chemie (149) | |||
3 | A, B, C, D | MAGNESIUM ION | non-polymer | 24.3 | 9 | Chemie (MG) | |||
4 | D, A, B, C | SODIUM ION | non-polymer | 23.0 | 16 | Chemie (NA) | |||
5 | A, B, C, D | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 94 | Chemie (DMS) | |||
6 | water | water | 18.0 | 2607 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 9 - 1023 (UniProt: P00722)
PDB | External Database | Details |
---|---|---|
Gly 1 | - | expression tag |
Ser 2 | - | expression tag |
His 3 | - | expression tag |
Met 4 | - | expression tag |
Leu 5 | - | expression tag |
Glu 6 | - | expression tag |
Asp 7 | - | expression tag |
Pro 8 | - | expression tag |
Thr 460 | Asn 461 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 465973.1 | |
Non-Polymers* | Number of molecules | 123 |
Total formula weight | 8643.6 | |
All* | Total formula weight | 474616.7 |