3CZJ
E. COLI (lacZ) BETA-GALACTOSIDASE (N460T) IN COMPLEX WITH D-GALCTOPYRANOSYL-1-ONE
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| A | 0004565 | molecular_function | beta-galactosidase activity |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0005990 | biological_process | lactose catabolic process |
| A | 0009341 | cellular_component | beta-galactosidase complex |
| A | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| A | 0030246 | molecular_function | carbohydrate binding |
| A | 0031420 | molecular_function | alkali metal ion binding |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| B | 0004565 | molecular_function | beta-galactosidase activity |
| B | 0005975 | biological_process | carbohydrate metabolic process |
| B | 0005990 | biological_process | lactose catabolic process |
| B | 0009341 | cellular_component | beta-galactosidase complex |
| B | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| B | 0030246 | molecular_function | carbohydrate binding |
| B | 0031420 | molecular_function | alkali metal ion binding |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0046872 | molecular_function | metal ion binding |
| C | 0000287 | molecular_function | magnesium ion binding |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| C | 0004565 | molecular_function | beta-galactosidase activity |
| C | 0005975 | biological_process | carbohydrate metabolic process |
| C | 0005990 | biological_process | lactose catabolic process |
| C | 0009341 | cellular_component | beta-galactosidase complex |
| C | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| C | 0030246 | molecular_function | carbohydrate binding |
| C | 0031420 | molecular_function | alkali metal ion binding |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0046872 | molecular_function | metal ion binding |
| D | 0000287 | molecular_function | magnesium ion binding |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| D | 0004565 | molecular_function | beta-galactosidase activity |
| D | 0005975 | biological_process | carbohydrate metabolic process |
| D | 0005990 | biological_process | lactose catabolic process |
| D | 0009341 | cellular_component | beta-galactosidase complex |
| D | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| D | 0030246 | molecular_function | carbohydrate binding |
| D | 0031420 | molecular_function | alkali metal ion binding |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE 149 A 2001 |
| Chain | Residue |
| A | ASP201 |
| A | ASN604 |
| A | HIS391 |
| A | GLU461 |
| A | MET502 |
| A | TYR503 |
| A | GLU537 |
| A | HIS540 |
| A | TRP568 |
| A | PHE601 |
| site_id | AC2 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE 149 B 2001 |
| Chain | Residue |
| B | ASP201 |
| B | HIS391 |
| B | GLU461 |
| B | MET502 |
| B | TYR503 |
| B | GLU537 |
| B | HIS540 |
| B | TRP568 |
| B | PHE601 |
| B | ASN604 |
| site_id | AC3 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE 149 C 2001 |
| Chain | Residue |
| C | ASP201 |
| C | HIS391 |
| C | GLU461 |
| C | MET502 |
| C | TYR503 |
| C | GLU537 |
| C | HIS540 |
| C | TRP568 |
| C | PHE601 |
| C | ASN604 |
| site_id | AC4 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE 149 D 2001 |
| Chain | Residue |
| D | ASP201 |
| D | HIS391 |
| D | GLU461 |
| D | MET502 |
| D | TYR503 |
| D | GLU537 |
| D | HIS540 |
| D | TRP568 |
| D | PHE601 |
| D | ASN604 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MG A 3001 |
| Chain | Residue |
| A | GLU416 |
| A | HIS418 |
| A | GLU461 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG A 3002 |
| Chain | Residue |
| A | ASP15 |
| A | ASN18 |
| A | GLN163 |
| A | ASP193 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE NA A 3101 |
| Chain | Residue |
| A | ASP201 |
| A | PHE601 |
| A | ASN604 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA A 3102 |
| Chain | Residue |
| A | PHE556 |
| A | TYR559 |
| A | PRO560 |
| A | LEU562 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA A 3103 |
| Chain | Residue |
| A | PHE931 |
| A | PRO932 |
| A | LEU967 |
| A | MET968 |
| A | THR970 |
| site_id | BC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA A 3104 |
| Chain | Residue |
| A | SER647 |
| A | ASP648 |
| A | ASN649 |
| A | GLU650 |
| A | LEU670 |
| site_id | BC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MG B 3001 |
| Chain | Residue |
| B | GLU416 |
| B | HIS418 |
| B | GLU461 |
| site_id | BC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG B 3002 |
| Chain | Residue |
| B | ASP15 |
| B | ASN18 |
| B | GLN163 |
| B | ASP193 |
| site_id | BC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE NA B 3101 |
| Chain | Residue |
| B | ASP201 |
| B | PHE601 |
| B | ASN604 |
| site_id | BC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA B 3102 |
| Chain | Residue |
| B | PHE556 |
| B | TYR559 |
| B | PRO560 |
| B | LEU562 |
| site_id | BC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA B 3103 |
| Chain | Residue |
| B | PHE931 |
| B | PRO932 |
| B | LEU967 |
| B | THR970 |
| site_id | BC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA B 3104 |
| Chain | Residue |
| B | SER647 |
| B | ASN649 |
| B | GLU650 |
| B | LEU670 |
| site_id | BC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MG C 3001 |
| Chain | Residue |
| C | GLU416 |
| C | HIS418 |
| C | GLU461 |
| site_id | CC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG C 3002 |
| Chain | Residue |
| C | ASP15 |
| C | ASN18 |
| C | GLN163 |
| C | ASP193 |
| site_id | CC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE NA C 3101 |
| Chain | Residue |
| C | ASP201 |
| C | PHE601 |
| C | ASN604 |
| site_id | CC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA C 3102 |
| Chain | Residue |
| C | LEU562 |
| C | PHE556 |
| C | TYR559 |
| C | PRO560 |
| site_id | CC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA C 3103 |
| Chain | Residue |
| C | PHE931 |
| C | PRO932 |
| C | LEU967 |
| C | THR970 |
| site_id | CC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA C 3104 |
| Chain | Residue |
| C | SER647 |
| C | ASN649 |
| C | GLU650 |
| C | LEU670 |
| site_id | CC6 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MG D 3001 |
| Chain | Residue |
| D | GLU416 |
| D | HIS418 |
| D | GLU461 |
| site_id | CC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG D 3002 |
| Chain | Residue |
| D | ASP15 |
| D | ASN18 |
| D | GLN163 |
| D | ASP193 |
| site_id | CC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE NA D 3101 |
| Chain | Residue |
| D | ASP201 |
| D | PHE601 |
| D | ASN604 |
| site_id | CC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA D 3102 |
| Chain | Residue |
| D | PHE556 |
| D | TYR559 |
| D | PRO560 |
| D | LEU562 |
| site_id | DC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA D 3103 |
| Chain | Residue |
| D | PHE931 |
| D | PRO932 |
| D | LEU967 |
| D | THR970 |
| site_id | DC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA D 3104 |
| Chain | Residue |
| D | SER647 |
| D | ASP648 |
| D | ASN649 |
| D | GLU650 |
| D | LEU670 |
| site_id | DC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE DMS A 8401 |
| Chain | Residue |
| A | THR229 |
| A | GLY331 |
| A | ARG333 |
| A | ASN449 |
| A | HIS450 |
| A | PRO451 |
| A | ARG482 |
| site_id | DC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS A 8402 |
| Chain | Residue |
| A | ARG557 |
| A | HIS622 |
| A | GLN623 |
| A | GLN628 |
| site_id | DC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS A 8403 |
| Chain | Residue |
| A | LYS380 |
| A | ASN383 |
| A | PHE626 |
| A | TYR642 |
| A | TRP708 |
| site_id | DC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE DMS A 8404 |
| Chain | Residue |
| A | PRO32 |
| A | PHE33 |
| A | ALA34 |
| A | TRP36 |
| A | ASP45 |
| A | ALA327 |
| site_id | DC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS A 8405 |
| Chain | Residue |
| A | GLY270 |
| A | THR271 |
| A | ARG292 |
| site_id | DC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS A 8407 |
| Chain | Residue |
| A | ARG505 |
| A | GLU508 |
| A | PRO1001 |
| A | GLN1008 |
| site_id | DC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS A 8408 |
| Chain | Residue |
| A | LEU54 |
| A | ASN55 |
| A | LEU125 |
| A | PHE127 |
| site_id | EC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS A 8409 |
| Chain | Residue |
| A | GLU334 |
| A | ARG336 |
| A | PRO480 |
| site_id | EC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE DMS A 8410 |
| Chain | Residue |
| A | PRO106 |
| A | PRO115 |
| site_id | EC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS A 8411 |
| Chain | Residue |
| A | PRO584 |
| A | TRP585 |
| A | SER586 |
| A | ARG973 |
| site_id | EC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE DMS A 8412 |
| Chain | Residue |
| A | GLY275 |
| A | THR290 |
| site_id | EC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS A 8413 |
| Chain | Residue |
| A | GLY593 |
| A | ASP594 |
| A | THR595 |
| site_id | EC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS A 8415 |
| Chain | Residue |
| A | LYS621 |
| A | ARG699 |
| A | ILE714 |
| A | TRP717 |
| site_id | EC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS D 8420 |
| Chain | Residue |
| A | ASP428 |
| D | GLN445 |
| D | TRP474 |
| site_id | EC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS A 8421 |
| Chain | Residue |
| A | HIS93 |
| A | GLY94 |
| A | TYR95 |
| site_id | EC9 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE DMS A 8501 |
| Chain | Residue |
| A | TRP36 |
| A | ASP45 |
| site_id | FC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS A 8502 |
| Chain | Residue |
| A | GLU57 |
| A | LEU125 |
| A | THR126 |
| site_id | FC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS A 8602 |
| Chain | Residue |
| A | GLN266 |
| A | ALA268 |
| A | SER269 |
| site_id | FC3 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE DMS A 8603 |
| Chain | Residue |
| A | ASN102 |
| site_id | FC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS A 8604 |
| Chain | Residue |
| A | GLU314 |
| A | HIS316 |
| A | GLY320 |
| site_id | FC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS A 8605 |
| Chain | Residue |
| A | ILE107 |
| A | THR108 |
| A | PRO596 |
| site_id | FC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS A 8606 |
| Chain | Residue |
| A | PHE60 |
| A | TRP62 |
| A | GLU80 |
| A | ASP82 |
| site_id | FC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS A 8607 |
| Chain | Residue |
| A | ARG37 |
| A | SER132 |
| A | TRP133 |
| A | GLU136 |
| A | HIS216 |
| site_id | FC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS B 8401 |
| Chain | Residue |
| B | GLY331 |
| B | ARG333 |
| B | ASN449 |
| B | PRO451 |
| B | ARG482 |
| site_id | FC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS B 8402 |
| Chain | Residue |
| B | ARG557 |
| B | HIS622 |
| B | GLN625 |
| B | GLN628 |
| B | GLN718 |
| site_id | GC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS B 8403 |
| Chain | Residue |
| B | LYS380 |
| B | ASN383 |
| B | PHE626 |
| B | TYR642 |
| B | TRP708 |
| site_id | GC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE DMS B 8404 |
| Chain | Residue |
| B | PRO32 |
| B | PHE33 |
| B | ALA34 |
| B | TRP36 |
| B | ASP45 |
| B | ALA327 |
| site_id | GC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS B 8405 |
| Chain | Residue |
| B | GLY270 |
| B | THR271 |
| B | LEU291 |
| B | ARG292 |
| site_id | GC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS B 8406 |
| Chain | Residue |
| B | GLU314 |
| B | HIS316 |
| B | GLY320 |
| B | LEU322 |
| site_id | GC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS B 8407 |
| Chain | Residue |
| B | ARG505 |
| B | GLU508 |
| B | PRO1001 |
| site_id | GC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS B 8408 |
| Chain | Residue |
| B | LEU54 |
| B | ASN55 |
| B | GLU57 |
| B | LEU125 |
| site_id | GC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS B 8409 |
| Chain | Residue |
| B | GLU334 |
| B | ARG336 |
| B | PRO480 |
| site_id | GC8 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE DMS B 8410 |
| Chain | Residue |
| B | PRO106 |
| site_id | GC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS B 8411 |
| Chain | Residue |
| B | PRO584 |
| B | TRP585 |
| B | SER586 |
| B | ARG973 |
| C | ASN581 |
| site_id | HC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS B 8412 |
| Chain | Residue |
| B | GLY275 |
| B | THR290 |
| B | ARG292 |
| site_id | HC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS B 8413 |
| Chain | Residue |
| B | GLY593 |
| B | ASP594 |
| B | THR595 |
| site_id | HC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS B 8420 |
| Chain | Residue |
| B | ASP428 |
| B | PRO430 |
| C | TRP474 |
| site_id | HC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS B 8421 |
| Chain | Residue |
| B | HIS93 |
| B | GLY94 |
| B | TYR95 |
| site_id | HC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS B 8425 |
| Chain | Residue |
| B | ASP648 |
| B | ASN649 |
| B | GLU650 |
| site_id | HC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE DMS B 8501 |
| Chain | Residue |
| B | ASP45 |
| B | ARG46 |
| site_id | HC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS B 8502 |
| Chain | Residue |
| B | GLU57 |
| B | TRP58 |
| B | LEU125 |
| B | THR126 |
| site_id | HC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS B 8503 |
| Chain | Residue |
| B | ASN102 |
| B | SER796 |
| B | TRP999 |
| site_id | HC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS B 8504 |
| Chain | Residue |
| B | PRO396 |
| B | LEU397 |
| B | THR400 |
| site_id | IC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE DMS B 8505 |
| Chain | Residue |
| B | SER53 |
| B | ASN55 |
| site_id | IC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE DMS B 8506 |
| Chain | Residue |
| B | SER923 |
| B | TYR962 |
| site_id | IC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS B 8508 |
| Chain | Residue |
| B | PHE60 |
| B | ALA61 |
| B | TRP62 |
| B | GLU80 |
| B | ASP82 |
| site_id | IC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS B 8509 |
| Chain | Residue |
| B | GLU41 |
| C | GLN49 |
| C | ARG52 |
| site_id | IC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS B 8510 |
| Chain | Residue |
| B | LEU377 |
| B | ALA707 |
| B | GLU710 |
| site_id | IC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE DMS C 8401 |
| Chain | Residue |
| C | THR229 |
| C | GLY331 |
| C | ARG333 |
| C | ASN449 |
| C | HIS450 |
| C | PRO451 |
| C | ARG482 |
| site_id | IC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS C 8402 |
| Chain | Residue |
| C | ARG557 |
| C | HIS622 |
| C | GLN623 |
| C | GLN628 |
| C | GLN718 |
| site_id | IC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS C 8403 |
| Chain | Residue |
| C | LYS380 |
| C | ASN383 |
| C | PHE626 |
| C | TYR642 |
| C | TRP708 |
| site_id | IC9 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE DMS C 8404 |
| Chain | Residue |
| C | PRO32 |
| C | PHE33 |
| C | ALA34 |
| C | TRP36 |
| C | ASP45 |
| C | ALA327 |
| site_id | JC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS C 8405 |
| Chain | Residue |
| C | THR271 |
| C | LEU291 |
| C | ARG292 |
| site_id | JC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS C 8406 |
| Chain | Residue |
| C | GLU314 |
| C | HIS316 |
| C | GLY320 |
| C | THR321 |
| C | LEU322 |
| site_id | JC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS C 8407 |
| Chain | Residue |
| C | ARG505 |
| C | GLU508 |
| C | PRO1001 |
| site_id | JC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS C 8408 |
| Chain | Residue |
| C | SER53 |
| C | LEU54 |
| C | ASN55 |
| C | LEU125 |
| site_id | JC5 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE DMS C 8409 |
| Chain | Residue |
| C | GLU334 |
| C | ARG336 |
| site_id | JC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS C 8411 |
| Chain | Residue |
| C | ILE576 |
| C | PRO584 |
| C | TRP585 |
| C | SER586 |
| C | ARG973 |
| site_id | JC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS C 8412 |
| Chain | Residue |
| C | GLY275 |
| C | GLU277 |
| C | THR290 |
| site_id | JC8 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE DMS C 8414 |
| Chain | Residue |
| C | HIS93 |
| site_id | JC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS C 8415 |
| Chain | Residue |
| C | LYS621 |
| C | ARG699 |
| C | TRP717 |
| site_id | KC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE DMS C 8420 |
| Chain | Residue |
| C | ASP428 |
| C | PRO430 |
| site_id | KC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS C 8421 |
| Chain | Residue |
| C | HIS93 |
| C | GLY94 |
| C | TYR95 |
| site_id | KC3 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE DMS C 8422 |
| Chain | Residue |
| C | PRO788 |
| C | MET968 |
| site_id | KC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS C 8425 |
| Chain | Residue |
| C | ASP648 |
| C | ASN649 |
| C | GLU650 |
| site_id | KC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS C 8501 |
| Chain | Residue |
| C | TRP36 |
| C | ASP45 |
| C | ARG46 |
| site_id | KC6 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS C 8503 |
| Chain | Residue |
| C | PHE629 |
| C | GLN718 |
| C | TRP720 |
| site_id | KC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE DMS C 8504 |
| Chain | Residue |
| C | ASN102 |
| C | SER796 |
| site_id | KC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS C 8505 |
| Chain | Residue |
| C | ILE305 |
| C | PRO306 |
| C | ASN307 |
| site_id | KC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS C 8506 |
| Chain | Residue |
| C | PHE608 |
| C | ASP610 |
| C | THR612 |
| C | ARG973 |
| site_id | LC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS C 8507 |
| Chain | Residue |
| B | GLN49 |
| C | PRO47 |
| C | SER48 |
| C | GLN49 |
| C | LEU51 |
| site_id | LC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE DMS D 8401 |
| Chain | Residue |
| D | THR229 |
| D | GLY331 |
| D | ARG333 |
| D | ASN449 |
| D | HIS450 |
| D | PRO451 |
| D | ARG482 |
| site_id | LC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS D 8402 |
| Chain | Residue |
| D | ARG557 |
| D | HIS622 |
| D | GLN623 |
| D | GLN628 |
| D | GLN718 |
| site_id | LC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS D 8403 |
| Chain | Residue |
| D | LYS380 |
| D | ASN383 |
| D | PHE626 |
| D | TYR642 |
| D | TRP708 |
| site_id | LC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE DMS D 8404 |
| Chain | Residue |
| D | PRO32 |
| D | PHE33 |
| D | ALA34 |
| D | TRP36 |
| D | ASP45 |
| D | ALA327 |
| site_id | LC6 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS D 8405 |
| Chain | Residue |
| D | GLY270 |
| D | THR271 |
| D | ARG292 |
| site_id | LC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS D 8407 |
| Chain | Residue |
| D | ARG505 |
| D | GLU508 |
| D | PRO1001 |
| site_id | LC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS D 8408 |
| Chain | Residue |
| D | SER53 |
| D | LEU54 |
| D | ASN55 |
| site_id | LC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS D 8409 |
| Chain | Residue |
| D | GLU334 |
| D | ARG336 |
| D | PRO480 |
| site_id | MC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS D 8411 |
| Chain | Residue |
| D | ILE576 |
| D | PRO584 |
| D | TRP585 |
| D | SER586 |
| D | ARG973 |
| site_id | MC2 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE DMS D 8412 |
| Chain | Residue |
| D | THR290 |
| site_id | MC3 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE DMS D 8414 |
| Chain | Residue |
| D | HIS93 |
| site_id | MC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS D 8415 |
| Chain | Residue |
| D | LYS621 |
| D | ARG699 |
| D | TRP717 |
| site_id | MC5 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE DMS D 8417 |
| Chain | Residue |
| D | ASN232 |
| D | GLU334 |
| site_id | MC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE DMS D 8501 |
| Chain | Residue |
| D | PRO47 |
| D | LEU51 |
| site_id | MC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS A 8704 |
| Chain | Residue |
| A | ARG282 |
| A | GLY284 |
| A | ALA286 |
| site_id | MC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS D 8705 |
| Chain | Residue |
| D | HIS418 |
| D | GLU461 |
| D | SER796 |
| D | TRP999 |
| site_id | MC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS D 8706 |
| Chain | Residue |
| D | TYR472 |
| D | THR494 |
| D | THR496 |
| D | ASP497 |
| site_id | NC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS A 8708 |
| Chain | Residue |
| A | GLN445 |
| A | TRP474 |
| D | ASP428 |
| D | PRO430 |
| site_id | NC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE DMS D 8709 |
| Chain | Residue |
| D | LEU341 |
| D | GLY346 |
Functional Information from PROSITE/UniProt
| site_id | PS00719 |
| Number of Residues | 26 |
| Details | GLYCOSYL_HYDROL_F2_1 Glycosyl hydrolases family 2 signature 1. NaVRCSHYPnhplWYtlcDryGLYVV |
| Chain | Residue | Details |
| A | ASN385-VAL410 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton donor => ECO:0000269|PubMed:6420154 |
| Chain | Residue | Details |
| A | GLU461 | |
| B | GLU461 | |
| C | GLU461 | |
| D | GLU461 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | ACT_SITE: Nucleophile => ECO:0000269|PubMed:1350782 |
| Chain | Residue | Details |
| A | GLU537 | |
| B | GLU537 | |
| C | GLU537 | |
| D | GLU537 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 28 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | ASN102 | |
| B | GLU461 | |
| B | GLU537 | |
| B | PHE601 | |
| B | ASN604 | |
| B | TRP999 | |
| C | ASN102 | |
| C | ASP201 | |
| C | GLU461 | |
| C | GLU537 | |
| C | PHE601 | |
| A | ASP201 | |
| C | ASN604 | |
| C | TRP999 | |
| D | ASN102 | |
| D | ASP201 | |
| D | GLU461 | |
| D | GLU537 | |
| D | PHE601 | |
| D | ASN604 | |
| D | TRP999 | |
| A | GLU461 | |
| A | GLU537 | |
| A | PHE601 | |
| A | ASN604 | |
| A | TRP999 | |
| B | ASN102 | |
| B | ASP201 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11045615 |
| Chain | Residue | Details |
| A | GLU416 | |
| D | GLU416 | |
| D | HIS418 | |
| D | ASN597 | |
| A | HIS418 | |
| A | ASN597 | |
| B | GLU416 | |
| B | HIS418 | |
| B | ASN597 | |
| C | GLU416 | |
| C | HIS418 | |
| C | ASN597 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 8 |
| Details | SITE: Transition state stabilizer |
| Chain | Residue | Details |
| A | HIS357 | |
| A | HIS391 | |
| B | HIS357 | |
| B | HIS391 | |
| C | HIS357 | |
| C | HIS391 | |
| D | HIS357 | |
| D | HIS391 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | SITE: Important for ensuring that an appropriate proportion of lactose is converted to allolactose |
| Chain | Residue | Details |
| A | TRP999 | |
| B | TRP999 | |
| C | TRP999 | |
| D | TRP999 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1bgl |
| Chain | Residue | Details |
| A | GLU461 | |
| A | GLU537 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1bgl |
| Chain | Residue | Details |
| B | GLU461 | |
| B | GLU537 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1bgl |
| Chain | Residue | Details |
| C | GLU461 | |
| C | GLU537 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1bgl |
| Chain | Residue | Details |
| D | GLU461 | |
| D | GLU537 |
| site_id | CSA5 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1bgl |
| Chain | Residue | Details |
| A | TYR503 | |
| A | GLU461 | |
| A | GLU537 |
| site_id | CSA6 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1bgl |
| Chain | Residue | Details |
| B | TYR503 | |
| B | GLU461 | |
| B | GLU537 |
| site_id | CSA7 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1bgl |
| Chain | Residue | Details |
| C | TYR503 | |
| C | GLU461 | |
| C | GLU537 |
| site_id | CSA8 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1bgl |
| Chain | Residue | Details |
| D | TYR503 | |
| D | GLU461 | |
| D | GLU537 |
| site_id | MCSA1 |
| Number of Residues | 10 |
| Details | M-CSA 422 |
| Chain | Residue | Details |
| A | ASP201 | |
| A | ASN604 | |
| A | HIS357 | |
| A | HIS391 | |
| A | GLU416 | |
| A | HIS418 | |
| A | GLU461 | |
| A | GLU537 | |
| A | ASN597 | |
| A | PHE601 |
| site_id | MCSA2 |
| Number of Residues | 10 |
| Details | M-CSA 422 |
| Chain | Residue | Details |
| B | ASP201 | |
| B | ASN604 | |
| B | HIS357 | |
| B | HIS391 | |
| B | GLU416 | |
| B | HIS418 | |
| B | GLU461 | |
| B | GLU537 | |
| B | ASN597 | |
| B | PHE601 |
| site_id | MCSA3 |
| Number of Residues | 10 |
| Details | M-CSA 422 |
| Chain | Residue | Details |
| C | ASP201 | |
| C | ASN604 | |
| C | HIS357 | |
| C | HIS391 | |
| C | GLU416 | |
| C | HIS418 | |
| C | GLU461 | |
| C | GLU537 | |
| C | ASN597 | |
| C | PHE601 |
| site_id | MCSA4 |
| Number of Residues | 10 |
| Details | M-CSA 422 |
| Chain | Residue | Details |
| D | ASP201 | |
| D | ASN604 | |
| D | HIS357 | |
| D | HIS391 | |
| D | GLU416 | |
| D | HIS418 | |
| D | GLU461 | |
| D | GLU537 | |
| D | ASN597 | |
| D | PHE601 |
