3CIG

Crystal structure of mouse TLR3 ectodomain

3CIG の概要

• エントリーDOI: 10.2210/pdb3cig/pdb
• 関連するPDBエントリー: 3CIY
• 分子名称: Toll-like receptor 3, 2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• 機能のキーワード: leucine-rich repeat, innate immunity, tlr, lrr, glycoprotein, immune response, inflammatory response, membrane, receptor, transmembrane, immune system
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 84591.96

構造登録者

Liu, L.,Botos, I.,Wang, Y.,Leonard, J.N.,Shiloach, J.,Segal, D.M.,Davies, D.R. (登録日: 2008-03-11, 公開日: 2008-05-06, 最終更新日: 2024-11-20)

主引用文献

Liu, L.,Botos, I.,Wang, Y.,Leonard, J.N.,Shiloach, J.,Segal, D.M.,Davies, D.R.
Structural basis of toll-like receptor 3 signaling with double-stranded RNA.
Science, 320:379-381, 2008

PubMed Abstract: Toll-like receptor 3 (TLR3) recognizes double-stranded RNA (dsRNA), a molecular signature of most viruses, and triggers inflammatory responses that prevent viral spread. TLR3 ectodomains (ECDs) dimerize on oligonucleotides of at least 40 to 50 base pairs in length, the minimal length required for signal transduction. To establish the molecular basis for ligand binding and signaling, we determined the crystal structure of a complex between two mouse TLR3-ECDs and dsRNA at 3.4 angstrom resolution. Each TLR3-ECD binds dsRNA at two sites located at opposite ends of the TLR3 horseshoe, and an intermolecular contact between the two TLR3-ECD C-terminal domains coordinates and stabilizes the dimer. This juxtaposition could mediate downstream signaling by dimerizing the cytoplasmic Toll interleukin-1 receptor (TIR) domains. The overall shape of the TLR3-ECD does not change upon binding to dsRNA.

PubMed: 18420935
DOI: 10.1126/science.1155406

実験手法

X-RAY DIFFRACTION (2.66 Å)