3CIG
Crystal structure of mouse TLR3 ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 70 |
Detector technology | CCD |
Collection date | 2007-11-29 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 82.681, 158.017, 185.278 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.660 |
R-factor | 0.24542 |
Rwork | 0.244 |
R-free | 0.27216 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a0z |
RMSD bond length | 0.008 |
RMSD bond angle | 1.758 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.108 | 0.455 |
Number of reflections | 33494 | |
<I/σ(I)> | 15.125 | 1.79 |
Completeness [%] | 96.2 | 90.7 |
Redundancy | 3.9 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 294 | 15% PEG 6000, 5% dextran 5000, 1M lithium chloride, 0.1M citric acid, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |