3B8X

Crystal structure of GDP-4-keto-6-deoxymannose-3-dehydratase (ColD) H188N mutant with bound GDP-perosamine

Summary for 3B8X

Related2GMU 2R0T
DescriptorPyridoxamine 5-phosphate-dependent dehydrase, SODIUM ION, [(2R,3S,4R,5R)-5-(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S,5S,6R)-3,4-dihydroxy-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-6-methyltetrahydro-2H-pyran-2-yl dihydrogen diphosphate, ... (5 entities in total)
Functional Keywordsaspartate aminotransferase, colitose, perosamine, o-antigen, plp, pyridoxal phosphate, transferase
Biological sourceEscherichia coli
Total number of polymer chains2
Total molecular weight90665.87
Authors
Cook, P.D.,Holden, H.M. (deposition date: 2007-11-02, release date: 2007-11-27, Last modification date: 2011-07-13)
Primary citation
Cook, P.D.,Holden, H.M.
GDP-4-Keto-6-deoxy-D-mannose 3-Dehydratase, Accommodating a Sugar Substrate in the Active Site.
J.Biol.Chem., 283:4295-4303, 2008
PubMed: 18045869 (PDB entries with the same primary citation)
DOI: 10.1074/jbc.M708893200
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.7 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliersRSRZ outliers11 0.4% 6.6%0MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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170172
PDB entries from 2020-10-21