3A6P

Crystal structure of Exportin-5:RanGTP:pre-miRNA complex

Summary for 3A6P

• Entry DOI: 10.2210/pdb3a6p/pdb
• Descriptor: Exportin-5, 13-mer peptide, GTP-binding nuclear protein Ran, ... (7 entities in total)
• Functional Keywords: exportin-5, pre-microrna, rangtp, nuclearexport, importin-beta family, nucleus, phosphoprotein, protein transport, rna-binding, rna-mediated gene silencing, transport, trna-binding, cell cycle, cell division, gtp-binding, isopeptide bond, mitosis, nucleotide-binding, protein transport-nuclear protein-rna complex, protein transport/nuclear protein/rna
• Biological source: Homo sapiens (human); More
• Cellular location: Nucleus : Q9HAV4 P62825
• Total number of polymer chains: 10
• Total formula weight: 355861.69

Authors

Okada, C.,Yamashita, E.,Lee, S.J.,Shibata, S.,Katahira, J.,Nakagawa, A.,Yoneda, Y.,Tsukihara, T. (deposition date: 2009-09-07, release date: 2009-12-08, Last modification date: 2024-11-13)

Primary citation

Okada, C.,Yamashita, E.,Lee, S.J.,Shibata, S.,Katahira, J.,Nakagawa, A.,Yoneda, Y.,Tsukihara, T.
A high-resolution structure of the pre-microRNA nuclear export machinery
Science, 326:1275-1279, 2009

PubMed Abstract: Nuclear export of microRNAs (miRNAs) by exportin-5 (Exp-5) is an essential step in miRNA biogenesis. Here, we present the 2.9 angstrom structure of the pre-miRNA nuclear export machinery formed by pre-miRNA complexed with Exp-5 and a guanine triphosphate (GTP)-bound form of the small nuclear guanine triphosphatase (GTPase) Ran (RanGTP). The x-ray structure shows that Exp-5:RanGTP recognizes the 2-nucleotide 3' overhang structure and the double-stranded stem of the pre-miRNA. Exp-5:RanGTP shields the pre-miRNA stem from degradation in a baseball mitt-like structure where it is held by broadly distributed weak interactions, whereas a tunnel-like structure of Exp-5 interacts strongly with the 2-nucleotide 3' overhang through hydrogen bonds and ionic interactions. RNA recognition by Exp-5:RanGTP does not depend on RNA sequence, implying that Exp-5:RanGTP can recognize a variety of pre-miRNAs.

PubMed: 19965479
DOI: 10.1126/science.1178705

Experimental method

X-RAY DIFFRACTION (2.92 Å)