3A6P

Crystal structure of Exportin-5:RanGTP:pre-miRNA complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-12
Detector	Bruker DIP-6040
Wavelength(s)	0.9000
Spacegroup name	P 1 21 1
Unit cell lengths	71.157, 304.667, 89.230
Unit cell angles	90.00, 110.79, 90.00

Refinement procedure

Resolution	39.840 - 2.920
R-factor	0.25061
Rwork	0.247
R-free	0.31242
Structure solution method	MAD
RMSD bond length	0.019
RMSD bond angle	1.828
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
High resolution limit [Å]	2.900
Number of reflections	74959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1