Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3A6P

Crystal structure of Exportin-5:RanGTP:pre-miRNA complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000049	molecular_function	tRNA binding
A	0003723	molecular_function	RNA binding
A	0003729	molecular_function	mRNA binding
A	0005049	molecular_function	nuclear export signal receptor activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006405	biological_process	RNA export from nucleus
A	0006611	biological_process	protein export from nucleus
A	0006886	biological_process	intracellular protein transport
A	0010586	biological_process	miRNA metabolic process
A	0015031	biological_process	protein transport
A	0016442	cellular_component	RISC complex
A	0031047	biological_process	regulatory ncRNA-mediated gene silencing
A	0031267	molecular_function	small GTPase binding
A	0035281	biological_process	pre-miRNA export from nucleus
A	0042565	cellular_component	RNA nuclear export complex
A	0070883	molecular_function	pre-miRNA binding
A	1905172	molecular_function	RISC complex binding
C	0000054	biological_process	ribosomal subunit export from nucleus
C	0000070	biological_process	mitotic sister chromatid segregation
C	0000287	molecular_function	magnesium ion binding
C	0003924	molecular_function	GTPase activity
C	0005049	molecular_function	nuclear export signal receptor activity
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005634	cellular_component	nucleus
C	0005635	cellular_component	nuclear envelope
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006606	biological_process	protein import into nucleus
C	0006611	biological_process	protein export from nucleus
C	0006913	biological_process	nucleocytoplasmic transport
C	0015031	biological_process	protein transport
C	0016442	cellular_component	RISC complex
C	0016787	molecular_function	hydrolase activity
C	0032991	cellular_component	protein-containing complex
C	0035281	biological_process	pre-miRNA export from nucleus
C	0042470	cellular_component	melanosome
C	0046039	biological_process	GTP metabolic process
C	0046827	biological_process	positive regulation of protein export from nucleus
C	0046872	molecular_function	metal ion binding
C	0051301	biological_process	cell division
C	0061015	biological_process	snRNA import into nucleus
C	0070883	molecular_function	pre-miRNA binding
C	1905172	molecular_function	RISC complex binding
F	0000049	molecular_function	tRNA binding
F	0003723	molecular_function	RNA binding
F	0003729	molecular_function	mRNA binding
F	0005049	molecular_function	nuclear export signal receptor activity
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006405	biological_process	RNA export from nucleus
F	0006611	biological_process	protein export from nucleus
F	0006886	biological_process	intracellular protein transport
F	0010586	biological_process	miRNA metabolic process
F	0015031	biological_process	protein transport
F	0016442	cellular_component	RISC complex
F	0031047	biological_process	regulatory ncRNA-mediated gene silencing
F	0031267	molecular_function	small GTPase binding
F	0035281	biological_process	pre-miRNA export from nucleus
F	0042565	cellular_component	RNA nuclear export complex
F	0070883	molecular_function	pre-miRNA binding
F	1905172	molecular_function	RISC complex binding
H	0000054	biological_process	ribosomal subunit export from nucleus
H	0000070	biological_process	mitotic sister chromatid segregation
H	0000287	molecular_function	magnesium ion binding
H	0003924	molecular_function	GTPase activity
H	0005049	molecular_function	nuclear export signal receptor activity
H	0005515	molecular_function	protein binding
H	0005525	molecular_function	GTP binding
H	0005634	cellular_component	nucleus
H	0005635	cellular_component	nuclear envelope
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006606	biological_process	protein import into nucleus
H	0006611	biological_process	protein export from nucleus
H	0006913	biological_process	nucleocytoplasmic transport
H	0015031	biological_process	protein transport
H	0016442	cellular_component	RISC complex
H	0016787	molecular_function	hydrolase activity
H	0032991	cellular_component	protein-containing complex
H	0035281	biological_process	pre-miRNA export from nucleus
H	0042470	cellular_component	melanosome
H	0046039	biological_process	GTP metabolic process
H	0046827	biological_process	positive regulation of protein export from nucleus
H	0046872	molecular_function	metal ion binding
H	0051301	biological_process	cell division
H	0061015	biological_process	snRNA import into nucleus
H	0070883	molecular_function	pre-miRNA binding
H	1905172	molecular_function	RISC complex binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	20
Details	BINDING SITE FOR RESIDUE GTP C 1177

Chain	Residue
C	ASP18
C	TYR39
C	THR42
C	GLY68
C	ASN122
C	LYS123
C	ASP125
C	ILE126
C	SER150
C	ALA151
C	LYS152
C	GLY19
C	MG1178
C	GLY20
C	GLY22
C	LYS23
C	THR24
C	THR25
C	GLU36
C	LYS37

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG C 1178

Chain	Residue
C	THR24
C	THR42
C	ASP65
C	GTP1177

site_id	AC3
Number of Residues	25
Details	BINDING SITE FOR RESIDUE GTP H 1177

Chain	Residue
H	ASP18
H	GLY19
H	GLY20
H	THR21
H	GLY22
H	LYS23
H	THR24
H	THR25
H	PHE35
H	GLU36
H	LYS37
H	LYS38
H	TYR39
H	THR42
H	ALA67
H	GLY68
H	GLN69
H	ASN122
H	LYS123
H	ASP125
H	ILE126
H	SER150
H	ALA151
H	LYS152
H	MG1178

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG H 1178

Chain	Residue
H	THR24
H	THR42
H	GTP1177

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269\|PubMed:15602554, ECO:0000269\|PubMed:15864302, ECO:0000269\|PubMed:19965479, ECO:0000269\|PubMed:9533885, ECO:0000269\|PubMed:9878368, ECO:0007744\|PDB:1BYU, ECO:0007744\|PDB:1WA5, ECO:0007744\|PDB:2BKU, ECO:0007744\|PDB:3A6P, ECO:0007744\|PDB:3RAN, ECO:0007744\|PDB:3W3Z, ECO:0007744\|PDB:5BXQ

Chain	Residue	Details
C	ASP18
C	ASN122
C	SER150
H	ASP18
H	ASN122
H	SER150
F	ARG718
F	GLN1045

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269\|PubMed:15602554, ECO:0000269\|PubMed:15864302, ECO:0000269\|PubMed:19965479, ECO:0007744\|PDB:1WA5, ECO:0007744\|PDB:2BKU, ECO:0007744\|PDB:3A6P, ECO:0007744\|PDB:3W3Z

Chain	Residue	Details
C	GLU36
H	GLU36

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269\|PubMed:15602554, ECO:0000269\|PubMed:15864302, ECO:0000269\|PubMed:19965479, ECO:0007744\|PDB:1WA5, ECO:0007744\|PDB:2BKU, ECO:0007744\|PDB:3W3Z

Chain	Residue	Details
C	GLY68
H	GLY68

site_id	SWS_FT_FI4
Number of Residues	2
Details	SITE: Essential for GTP hydrolysis => ECO:0000250\|UniProtKB:P62826

Chain	Residue	Details
C	GLN69
H	GLN69

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: N-acetylalanine => ECO:0000250\|UniProtKB:P62826

Chain	Residue	Details
C	ALA2
H	ALA2

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0000250\|UniProtKB:P62826

Chain	Residue	Details
C	THR24
H	THR24

site_id	SWS_FT_FI7
Number of Residues	8
Details	MOD_RES: N6-acetyllysine => ECO:0000250\|UniProtKB:P62826

Chain	Residue	Details
C	LYS37
C	LYS60
C	LYS99
C	LYS134
H	LYS37
H	LYS60
H	LYS99
H	LYS134

site_id	SWS_FT_FI8
Number of Residues	2
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0000250\|UniProtKB:P62826

Chain	Residue	Details
C	LYS71
H	LYS71

site_id	SWS_FT_FI9
Number of Residues	2
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250\|UniProtKB:P62827

Chain	Residue	Details
C	LYS159
H	LYS159

site_id	SWS_FT_FI10
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250\|UniProtKB:P62826

Chain	Residue	Details
C	LYS71
H	LYS71

site_id	SWS_FT_FI11
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0000250\|UniProtKB:P62826

Chain	Residue	Details
C	LYS152
H	LYS152

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
C	GLN69

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
H	GLN69

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)