3CSY
Crystal structure of the trimeric prefusion Ebola virus glycoprotein in complex with a neutralizing antibody from a human survivor
Summary for 3CSY
| Entry DOI | 10.2210/pdb3csy/pdb |
| Descriptor | Fab KZ52 heavy chain, Fab KZ52 light chain, Envelope glycoprotein GP1, ... (8 entities in total) |
| Functional Keywords | glycoprotein-antibody complex, immune system-viral protein complex, immune system/viral protein |
| Biological source | Homo sapiens (human) More |
| Cellular location | GP2: Virion membrane; Single-pass type I membrane protein. GP1: Virion membrane; Peripheral membrane protein. GP2-delta: Secreted: Q05320 GP2: Virion membrane; Single-pass type I membrane protein (By similarity). GP1: Virion membrane; Peripheral membrane protein (By similarity). GP2-delta: Secreted (By similarity): P87666 |
| Total number of polymer chains | 16 |
| Total formula weight | 406070.53 |
| Authors | Lee, J.E.,Fusco, M.L.,Hessell, A.J.,Oswald, W.B.,Burton, D.R.,Saphire, E.O. (deposition date: 2008-04-10, release date: 2008-07-08, Last modification date: 2024-10-30) |
| Primary citation | Lee, J.E.,Fusco, M.L.,Hessell, A.J.,Oswald, W.B.,Burton, D.R.,Saphire, E.O. Structure of the Ebola virus glycoprotein bound to an antibody from a human survivor. Nature, 454:177-182, 2008 Cited by PubMed Abstract: Ebola virus (EBOV) entry requires the surface glycoprotein (GP) to initiate attachment and fusion of viral and host membranes. Here we report the crystal structure of EBOV GP in its trimeric, pre-fusion conformation (GP1+GP2) bound to a neutralizing antibody, KZ52, derived from a human survivor of the 1995 Kikwit outbreak. Three GP1 viral attachment subunits assemble to form a chalice, cradled by the GP2 fusion subunits, while a novel glycan cap and projected mucin-like domain restrict access to the conserved receptor-binding site sequestered in the chalice bowl. The glycocalyx surrounding GP is likely central to immune evasion and may explain why survivors have insignificant neutralizing antibody titres. KZ52 recognizes a protein epitope at the chalice base where it clamps several regions of the pre-fusion GP2 to the amino terminus of GP1. This structure provides a template for unravelling the mechanism of EBOV GP-mediated fusion and for future immunotherapeutic development. PubMed: 18615077DOI: 10.1038/nature07082 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.4 Å) |
Structure validation
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