2ZME
Integrated structural and functional model of the human ESCRT-II complex
Summary for 2ZME
Entry DOI | 10.2210/pdb2zme/pdb |
Related | 3CUQ |
Descriptor | Vacuolar-sorting protein SNF8, Vacuolar protein-sorting-associated protein 36, Vacuolar protein-sorting-associated protein 25, ... (4 entities in total) |
Functional Keywords | escrt, sorting, mbv, vps, nucleus, protein transport, transcription, transcription regulation, transport, endosome, lipid-binding |
Biological source | Homo sapiens (human) More |
Cellular location | Cytoplasm: Q96H20 Q86VN1 Q9BRG1 |
Total number of polymer chains | 4 |
Total formula weight | 80133.12 |
Authors | Im, Y.J.,Hurley, J.H. (deposition date: 2008-04-17, release date: 2008-11-04, Last modification date: 2023-11-01) |
Primary citation | Im, Y.J.,Hurley, J.H. Integrated structural model and membrane targeting mechanism of the human ESCRT-II complex Dev.Cell, 14:902-913, 2008 Cited by PubMed Abstract: ESCRT-II plays a pivotal role in receptor downregulation and multivesicular body biogenesis and is conserved from yeast to humans. The crystal structures of two human ESCRT-II complex structures have been determined at 2.6 and 2.9 A resolution, respectively. The complex has three lobes and contains one copy each of VPS22 and VPS36 and two copies of VPS25. The structure reveals a dynamic helical domain to which both the VPS22 and VPS36 subunits contribute that connects the GLUE domain to the rest of the ESCRT-II core. Hydrodynamic analysis shows that intact ESCRT-II has a compact, closed conformation. ESCRT-II binds to the ESCRT-I VPS28 C-terminal domain subunit through a helix immediately C-terminal to the VPS36-GLUE domain. ESCRT-II is targeted to endosomal membranes by the lipid-binding activities of both the Vps36 GLUE domain and the first helix of Vps22. These data provide a unifying structural and functional framework for the ESCRT-II complex. PubMed: 18539118DOI: 10.1016/j.devcel.2008.04.004 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.9 Å) |
Structure validation
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