2ZME
Integrated structural and functional model of the human ESCRT-II complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2007-06-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 81.480, 81.480, 226.244 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.740 - 2.900 |
R-factor | 0.21928 |
Rwork | 0.214 |
R-free | 0.31277 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cuq |
RMSD bond length | 0.016 |
RMSD bond angle | 1.728 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 19408 | |
<I/σ(I)> | 41.3 | 3.25 |
Completeness [%] | 99.6 | 99.5 |
Redundancy | 4.3 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 300 | 40% PEG300, 0.1M Tris-HCl, pH8.5, VAPOR DIFFUSION, SITTING DROP |